Multiplex Assay Kit for Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay)

GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 82-102 96
heparin plasma(n=5) 80-94 80

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Somatostatin (SST) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-101% 80-90% 83-95% 98-105%
EDTA plasma(n=5) 81-98% 78-103% 89-96% 78-89%
heparin plasma(n=5) 85-98% 97-104% 91-98% 84-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SST) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
JOP. Journal of the Pancreas Pancreatic Somatostatinoma Diagnosed Preoperatively: Report of a Case Pubmed: 24413789
PloS one Circadian Modulation of Anxiety: A Role for Somatostatin in the Amygdala Pubmed: 24376834
Peptides Enhanced expression of neuropeptide S (NPS) receptor in eosinophils from severe asthmatics and subjects with total IgE above 100IU/ml Pubmed:24239856
Journal of Endocrinology Inhibitory effect of somatostatin on insulin secretion is not mediated via the CNS Pubmed:25630331
Pharmacol Res Oral curcumin has anti-arthritic efficacy through somatostatin generation PubMed: 25836921
Acta Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat pubmed:27685769
Applied Biological Chemistry Positive enhancement of Lactobacillus fermentum HY01 on intestinal movements of mice having constipation 10.1007/s13765-017-0327-3
Biomed Research International Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation pubmed:29082242
Molecular Neurobiology Somatostatin Maintains Permeability and Integrity of Blood-Brain Barrier in β-Amyloid Induced Toxicity Pubmed:29700775
Evidence-Based Complementary and Alternative Medicine Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids Pubmed:29853946
Pancreatology Relationship between somatostatin and interleukin-6: A cross-sectional study in patients with acute pancreatitis Pubmed: 30279074
Cell Reports Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice Pubmed: 30404008
Science of Food and Agriculture Protective Effect of Silkworm Pupa Oil on Hydrochloric Acid/Ethanol‐Induced Gastric Ulcer Pubmed: 30479041
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
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KSA592Mu11 ELISA Kit DIY Materials for Somatostatin (SST) Main materials for "Do It (ELISA Kit) Yourself".