Multiplex Assay Kit for Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay)

C1-q; Autoantigen Clq

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-101 98
EDTA plasma(n=5) 90-103 97
heparin plasma(n=5) 96-104 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement 1q (C1q) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 82-90% 79-103% 94-105%
EDTA plasma(n=5) 84-103% 98-105% 92-105% 84-104%
heparin plasma(n=5) 94-101% 91-105% 92-101% 81-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:C1q) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. PubMed: 22941511
Fish and Shellfish Immunology Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity Pubmed: 23085472
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
Archives of Gynecology and Obstetrics The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosis Pubmed:29572748
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
PLoS One Complement activation profile of patients with primary focal segmental glomerulosclerosis Pubmed: 32569286
CELL Liver Immune Profiling Reveals Pathogenesis and Therapeutics for Biliary Atresia 33248023
Chinese Journal of Nephrology, Dialysis & Transplantation Plasma and urine complement activation products in light chain and heavy chain deposition disease
Journal of Chemical Neuroanatomy Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity 34280490
Neuropsychiatr Dis Treat Elevated Serum Complement C1q Levels After Traumatic Brain Injury and Its Association with Poor Prognosis Pubmed:35035218
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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