Magnetic Luminex Assay Kit for Beta-Endorphin (bEP) ,etc.


(Note: Up to 8-plex in one testing reaction)


This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP) ,etc..
No significant cross-reactivity or interference between Beta-Endorphin (bEP) ,etc. and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-97 88
EDTA plasma(n=5) 86-95 90
heparin plasma(n=5) 78-101 87


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-104% 81-93% 80-89% 91-99%
EDTA plasma(n=5) 95-105% 81-105% 81-95% 84-98%
heparin plasma(n=5) 84-102% 82-105% 83-101% 78-95%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:bEP) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.



Magazine Citations
Experimental Dermatology Endogenous μ-opioid peptides modulate immune response towards malignant melanoma Publish: RD10279
European Journal of Physiology Physiology and cell biology of acupuncture observed in calcium signaling activated by acoustic shear wave SpringerLink: tv015l3126833315
International Journal of Pharmaceutical Science Invention ISSN(Online) Effect of Ramadan fasting on endorphin and endocannabinoid level in serum, PBMC and macrophage Ijpsi: Source
Neurological Sciences Evaluation of immune parameters in chronic migraine with medication overuse Pubmed:24867859
Brain Behav. Cerebrospinal fluid and plasma b-endorphin levels in children with cerebral malaria. pubmed:28413714
Journal of Cosmet Dermatol. Ultraviolet A photosensitivity profile of dexchlorpheniramine maleate and promethazine-based creams: Anti-inflammatory,antihistaminic, and skin barrier protection properties DOI: 10.1111/jocd.12349
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial Pubmed:29512230
Journal of Pediatric Neurosciences Plasma and cerebrospinal fluid beta-endorphin levels show a strong association in children with cerebral malaria
Chinese Journal of Physiology Blood dopamine level enhanced by caffeine in men after treadmill running Pubmed: 31793465
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JOURNAL OF AFFECTIVE DISORDERS Pain Sensitivity and Plasma Beta-Endorphin in Adolescent Non-Suicidal Self-Injury Pubmed: 32961416
EUROPEAN NEUROPSYCHOPHARMACOLOGY Serum β-endorphin levels are associated with addiction to suicidal behavior: a pilot study Pubmed: 32855024
J Burn Care Res Efficacy of Acupuncture on Pain Mechanisms, Inflammatory Responses, and Wound Healing in the Acute Phase of Major Burns: An Experimental Study on Rats 34309681
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