Magnetic Luminex Assay Kit for Beta-Endorphin (bEP) ,etc.
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA806Eq
- Organism SpeciesEquus caballus; Equine (Horse) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length1.5h
- Detection Range0.98-1000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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- Add to Cart (96T)
This assay has high sensitivity and excellent specificity for detection of Beta-Endorphin (bEP) ,etc..
No significant cross-reactivity or interference between Beta-Endorphin (bEP) ,etc. and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Beta-Endorphin (bEP) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Beta-Endorphin (bEP) ,etc. in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-Endorphin (bEP) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-Endorphin (bEP) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Beta-Endorphin (bEP) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|96-well plate||1||Plate sealer for 96 wells||4|
|Pre-Mixed Standard||2||Standard Diluent||1×20mL|
|Pre-Mixed Magnetic beads (22#:bEP)||1||Analysis buffer||1×20mL|
|Pre-Mixed Detection Reagent A||1×120μL||Assay Diluent A||1×12mL|
|Detection Reagent B (PE-SA)||1×120μL||Assay Diluent B||1×12mL|
|Sheath Fluid||1×10mL||Wash Buffer (30 × concentrate)||1×20mL|
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
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|Catalog No.||Related products for research use of Equus caballus; Equine (Horse) Organism species||Applications (RESEARCH USE ONLY!)|
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