Multiplex Assay Kit for Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay)

NP-Y; PYY4; CPON; Pro-neuropeptide Y; Neuropeptide tyrosine; C-flanking peptide of NPY

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-105 99
EDTA plasma(n=5) 89-103 95
heparin plasma(n=5) 96-104 99
sodium citrate plasma(n=5) 87-104 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Neuropeptide Y (NPY) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 86-101% 87-103% 80-91%
EDTA plasma(n=5) 79-96% 90-97% 91-105% 84-91%
heparin plasma(n=5) 78-105% 97-104% 90-97% 81-88%
sodium citrate plasma(n=5) 87-95% 98-105% 95-102% 78-92%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NPY) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Hemodialysis International Is there any interaction of resistin and adiponectin levels with protein‐energy wasting among patients with chronic kidney disease Onlinelibrary: hdi.12072
Journal of Pediatric Endocrinology and Metabolism Changes in growth pattern, leptin ghrelin and neuropeptide Y levels after adenotonsillectomy in prepubertal children Degruyter: Source
Ren Fail. The role of adipocytokines on depressive symptoms of patients with chronic kidney disease Pubmed: 23879728
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development. Pubmed: 24077326
Coronary Artery Disease The relationship of plasma neuropeptide Y levels with coronary collateral development Lww:Source
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate Pubmed:25623392
J Neurosurg Anesthesiol High Plasma Levels of Neuropeptide Y Correlate With Good Clinical Outcome But are not Correlated to Cerebral Blood Flow or Vasospasm After Subarachnoid Hemorrhage PubMed: 25955865
Journal of Diabetes Research High Intensity Aerobic Exercise Training Improves Deficits of Cardiovascular Autonomic Function in a Rat Model of Type 1 Diabetes Mellitus with Moderate … Pubmed:26885531
Archives of Gynecology and Obstetrics Binge eating and biochemical markers of appetite in new users of the contraceptive depot medroxyprogesterone acetate pubmed:27604241
basic & clinical pharmacology & toxicology Effects of Di-isononyl Phthalate on Neuropeptide Y Expression in Differentiating Human Neuronal Cells. pubmed:27625336
Acta Neurobiologiae Experimentalis Aberrant changes of somatostatin and neuropeptide Y in brain of a genetic rat model for epilepsy: tremor rat pubmed:27685769
Cardiovascular research Nur77 protects against adverse cardiac remodelling by limiting Neuropeptide Y signalling in the sympathoadrenal-cardiac axis Pubmed:29850786
Journal of Periodontology Salivary neuropeptides, stress, and periodontitis Pubmed:28914594
Neuropeptides Mechanisms of sustained long-term weight loss after RYGB: α-MSH is a key factor Pubmed:29685637
Pharmacology Melatonin Induces Osteoblastic Differentiation of Mesenchymal Stem Cells and Promotes Fracture Healing in a Rat Model of Femoral Fracture via Neuropeptide Y … Pubmed: 30227410
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome
Marmara Medical Journal Investigation of the relationship between insulin resistance and neuropeptide Y levels in polycystic ovary syndrome.
neurochemical research Sevoflurane Induces Hippocampal Neuronal Apoptosis by Altering the Level of Neuropeptide Y in Neonatal Rats. Pubmed: 32378074
Hum Psychopharmacol Serum levels of neuropeptide Y in patients with chronic schizophrenia during treatment augmentation with sarcosine (results of the double©\blind randomized?¡­ 33245168
Arch Toxicol Serotonin involvement in okadaic acid-induced diarrhoea in vivo 34148100
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