Multiplex Assay Kit for Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay)

BCEI; HP1.A; HPS2; PS2; PNR2; Breast Cancer,Estrogen-Inducible Sequence Expressed In; Breast cancer estrogen-inducible protein; Polypeptide P1.A

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-97 94
EDTA plasma(n=5) 91-105 99
heparin plasma(n=5) 94-103 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-102% 80-92% 82-99% 97-105%
EDTA plasma(n=5) 89-96% 90-101% 80-98% 83-99%
heparin plasma(n=5) 80-94% 95-103% 86-102% 93-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:TFF1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
BMC Gastroenterology Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China Biomedcentral: Source
Biochimica et Biophysica Acta miR218-5p regulates the proliferation of gastric cancer cells by targeting TFF1 in an Erk1/2-dependent manner Pubmed:25652124
clinical cancer research Identification of a Three-Biomarker Panel in Urine for Early Detection of Pancreatic Adenocarcinoma PubMed: 26240291
BMC Cancer miR-632 promotes gastric cancer progression by accelerating angiogenesis in a TFF1-dependent manner Pubmed: 30612555
Nutrients Protective Effects of Wheat Peptides against Ethanol-Induced Gastric Mucosal Lesions in Rats: Vasodilation and Anti-Inflammation Pubmed: 32784583
International Journal of Molecular Sciences Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
PLOS MEDICINE A combination of urinary biomarker panel and PancRISK score for earlier detection of pancreatic cancer: A case¨Ccontrol study 33301466
Int. J. Mol. Sci. Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB049Mu01 Recombinant Trefoil Factor 1 (TFF1) Positive Control; Immunogen; SDS-PAGE; WB.
PAB049Mu01 Polyclonal Antibody to Trefoil Factor 1 (TFF1) WB; IHC; ICC; IP.
SEB049Mu ELISA Kit for Trefoil Factor 1 (TFF1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB049Mu Multiplex Assay Kit for Trefoil Factor 1 (TFF1) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB049Mu01 ELISA Kit DIY Materials for Trefoil Factor 1 (TFF1) Main materials for "Do It (ELISA Kit) Yourself".