Multiplex Assay Kit for Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 95-104 101
EDTA plasma(n=5) 88-99 92
heparin plasma(n=5) 79-96 86
sodium citrate plasma(n=5) 84-101 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Low Density Lipoprotein (LDL) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 88-98% 84-94% 89-103%
EDTA plasma(n=5) 97-105% 86-94% 97-105% 81-103%
heparin plasma(n=5) 93-102% 78-101% 95-103% 96-104%
sodium citrate plasma(n=5) 80-88% 79-96% 87-96% 88-95%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:LDL) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
PloS one Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL. NCBI: PMC3896367
PLoS One. Atheroprotective effect of oleoylethanolamide (OEA) targeting oxidized LDL Pubmed:24465540
J Lipid Res PCSK9 deficiency unmasks a sex-and tissue-specific subcellular distribution of the LDL and VLDL receptors in mice PubMed: 26323289
Neuroscience 27-Hydroxycholesterol contributes to disruptive effects on learning and memory by modulating cholesterol metabolism in the rat brain PubMed: 25987203
The Journal of Nutritional Biochemistry Protective role of n6/n3 PUFA supplementation with varying DHA/EPA ratios against atherosclerosis in mice Pubmed:27142749
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Int J Mol Sci. Combined Effects of Curcumin and Lycopene or Bixin in Yoghurt on Inhibition of LDL Oxidation and Increases in HDL and Paraoxonase Levels in Streptozotocin-Diabetic Rats. pubmed:28333071
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Microvascular Research Effects of insulin analogs as an add-on to metformin on cutaneous microcirculation in type 2 diabetic patients pubmed:28954218
Biochemical and Biophysical Research Communications Oxidized low-density lipoprotein promotes osteoclast differentiation from CD68 positive mononuclear cells by regulating HMGB1 release pubmed:29146189
Journal of Nutritional Science and Vitaminology (Tokyo) Anthocyanin Cyanidin-3-Glucoside Attenuates Platelet Granule Release in Mice Fed High-Fat Diets. pubmed:28978870
Environmental Science and Pollution Research Bifenthrin exerts proatherogenic effects via arterial accumulation of native and oxidized LDL in rats: the beneficial role of vitamin E and selenium Pubmed: 30465240
American Journal of Translational Research Alteration of circulating innate lymphoid cells in patients with atherosclerotic cerebral infarction Pubmed: 30662674
Nature Communications ER-residential Nogo-B accelerates NAFLD-associated HCC mediated by metabolic reprogramming of oxLDL lipophagy Pubmed: 31358770
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Ecotoxicol Environ Saf High-fat diet-induced aggravation of cardiovascular impairment in permethrin-treated Wistar rats 34224971
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