Multiplex Assay Kit for Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay)

GSR; GLUR; GRD1; Glutathione reductase, mitochondrial

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-95 91
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 84-91 88
sodium citrate plasma(n=5) 94-101 98

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 97-104% 91-101% 96-103%
EDTA plasma(n=5) 81-89% 97-105% 99-105% 96-103%
heparin plasma(n=5) 90-104% 80-98% 94-105% 80-99%
sodium citrate plasma(n=5) 79-101% 92-103% 79-102% 82-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:GR) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Oxidative Medicine and Cellular Longevity Doxorubicin Toxicity can be Ameliorated during Antioxidant L-Carnitine Supplementation PubMed: PMC3154045
PLoS One. P2×7?Receptor in the Kidneys of Diabetic Rats Submitted to Aerobic Training or to N-Acetylcysteine Supplementation Plos:Source
Diabetes/Metabolism Research and Reviews Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … doi:10.1002
Nutrition Journal Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study Pubmed:27071648
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder science:article
Pharmacological Reports The role of PON-1, GR, IL-18, and OxLDL in depression with and without posttraumatic stress disorder. pubmed:28623707
Canadian journal of physiology and pharmacology Ambroxol attenuates cisplatin-induced hepato and nephrotoxicity via inhibition of p-JNK/p-ERK Pubmed: 30383980
FREE RADICAL RESEARCH Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide … Pubmed: 32985285
Lipids Health Dis Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … Pubmed: 32487177
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … Pubmed: 32509152
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY TNFα Mediates the Interaction of Telomeres and Mitochondria Induced by Hyperglycemia: A Rural Community-Based Cross-Sectional Study Pubmed: 32454945
oxidative medine cellular longevity Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … Pubmed: 32617139
The Journal of Clinical Endocrinology & Metabolism High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα 34562085
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB314Mu01 Recombinant Glutathione Reductase (GR) Positive Control; Immunogen; SDS-PAGE; WB.
RPB314Mu02 Recombinant Glutathione Reductase (GR) Positive Control; Immunogen; SDS-PAGE; WB.
PAB314Mu01 Polyclonal Antibody to Glutathione Reductase (GR) WB; IHC; ICC; IP.
PAB314Mu02 Polyclonal Antibody to Glutathione Reductase (GR) WB; IHC; ICC; IP.
SEB314Mu ELISA Kit for Glutathione Reductase (GR) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB314Mu Multiplex Assay Kit for Glutathione Reductase (GR) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.