Multiplex Assay Kit for Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay)

CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL; Semaphorin 7A,GPI Membrane Anchor; John Milton Hagen Blood Group; Semaphorin-K1; Semaphorin-L

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-99 95
EDTA plasma(n=5) 95-105 101
heparin plasma(n=5) 93-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 85-101% 80-93% 79-94% 88-96%
EDTA plasma(n=5) 90-98% 78-93% 87-96% 84-102%
heparin plasma(n=5) 78-105% 98-105% 81-99% 79-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SEMA7A) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
PLOS ONE Semaphorin 7A Aggravates Pulmonary Inflammation during Lung Injury pmc:PMC4720127
Experimental and Therapeutic Medicine Anti-Semaphorin-7A single chain antibody demonstrates beneficial effects on pulmonary inflammation during acute lung injury Pubmed:29456642
Nature Communications Red blood cell-derived semaphorin 7A promotes thrombo-inflammation in myocardial ischemia-reperfusion injury through platelet GPIb Pubmed: 32161256
American Journal of Hematology Semaphorin 7A: A novel marker of disease activity in Gaucher disease Pubmed: 31990411
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB448Mu02 Recombinant Semaphorin 7A (SEMA7A) Positive Control; Immunogen; SDS-PAGE; WB.
RPB448Mu01 Recombinant Semaphorin 7A (SEMA7A) Positive Control; Immunogen; SDS-PAGE; WB.
PAB448Mu01 Polyclonal Antibody to Semaphorin 7A (SEMA7A) WB; IHC; ICC; IP.
PAB448Mu02 Polyclonal Antibody to Semaphorin 7A (SEMA7A) WB; IHC; ICC; IP.
SEB448Mu ELISA Kit for Semaphorin 7A (SEMA7A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB448Mu Multiplex Assay Kit for Semaphorin 7A (SEMA7A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.