Multiplex Assay Kit for N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay)

CDw325; CD325; CDHN; CDH2; Cadherin 2 Type 1; N-Cadherin

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-97 93
EDTA plasma(n=5) 92-105 98
heparin plasma(n=5) 89-97 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-88% 86-94% 78-93% 91-101%
EDTA plasma(n=5) 84-99% 92-101% 87-95% 82-98%
heparin plasma(n=5) 78-95% 91-105% 88-96% 97-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:NCAD) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Toxicology Letters Biomarkers for methotrexate-induced liver injury: urinary protein profiling of psoriasis patients. Pubmed: 23830989
British journal of haematology Circulating N-cadherin levels are a negative prognostic indicator in patients with multiple myeloma. Pubmed: 23438504
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY Elevated Cerebrospinal Fluid and Plasma N-Cadherin in Alzheimer Disease Pubmed: 32296844
Cell Biol Toxicol ZNF471 modulates EMT and functions as methylation regulated tumor suppressor with diagnostic and prognostic significance in cervical cancer 33566221
CELL BIOLOGY AND TOXICOLOGY Metastatic suppression by DOC2B is mediated by inhibition of epithelial-mesenchymal transition and induction of senescence 33758996
Pharmacol Res DOC2B is a Negative Regulator of Wnt/β-catenin Signaling Pathway in Cervical Cancer Pubmed:35500882
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPB481Mu01 Recombinant N-cadherin (NCAD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB481Mu02 Recombinant N-cadherin (NCAD) Positive Control; Immunogen; SDS-PAGE; WB.
RPB481Mu03 Recombinant N-cadherin (NCAD) Positive Control; Immunogen; SDS-PAGE; WB.
PAB481Mu02 Polyclonal Antibody to N-cadherin (NCAD) WB; IHC; ICC; IP.
PAB481Mu01 Polyclonal Antibody to N-cadherin (NCAD) WB; IHC; ICC; IP.
PAB481Mu03 Polyclonal Antibody to N-cadherin (NCAD) WB; IHC; ICC; IP.
LAB481Mu71 Biotin-Linked Polyclonal Antibody to N-cadherin (NCAD) WB; IHC; ICC.
SEB481Mu ELISA Kit for N-cadherin (NCAD) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB481Mu Multiplex Assay Kit for N-cadherin (NCAD) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.