Multiplex Assay Kit for Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay)

FABP-1; FABPL; L-FABP; LFABP; Liver-type fatty acid-binding protein; Fatty Acid Binding Protein 1, Liver

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 80-95 87
heparin plasma(n=5) 90-101 94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fatty Acid Binding Protein 1 (FABP1) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-91% 95-102% 80-95% 81-95%
EDTA plasma(n=5) 79-92% 93-101% 80-102% 80-97%
heparin plasma(n=5) 97-105% 87-102% 84-95% 99-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:FABP1) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
J Korean Med Sci. Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease Pubmed:25120320
Cryobiology.? Ex vivo use of a Rho-kinase inhibitor during renal preservation improves graft function upon reperfusion Pubmed:25555715
Clin Transl Sci Controlled Rewarming after Hypothermia: Adding a New Principle to Renal Preservation PubMed: 26053383
SAGE journals The role of serum I-FABP concentration in assessment of small intestine mucosa among HIV-infected patients Content: Early
Transplant International Kidney transplantation after oxygenated machine perfusion preservation with Custodiol‐N solution PubMed: 25882869
European Journal of Clinical Investigation Prediction of renal function upon reperfusion by ex situ controlled oxygenated rewarming pubmed:27718228
Journal of Clinical and Diagnostic Research  The Importance of Liver-Fatty Acid Binding Protein in Diagnosis of Liver Damage in Patients with Acute Hepatitis pubmed:28571184
Annals of Nutrition and Metabolism Plasma Free Fatty Acids and their Binding Proteins in Preterm Infants Pubmed:30045009
Scandinavian Journal of Clinical and Laboratory Investigation Isolated kidney perfusion: the influence of pulsatile flow Pubmed:29301417
International Journal of Medical Sciences Circulating fatty acid-binding protein 1 (FABP1) and nonalcoholic fatty liver disease in patients with type 2 diabetes mellitus Pubmed: 32038102
International Journal of Medical Sciences FABP1 and FABP2 as markers of diabetic nephropathy Pubmed: 32922200
Archives of Clinical and Biomedical Research Profile of Intestinal Barrier Functional Markers in Italian Patients with Diarrhea-Predominant IBS: Preliminary Data from a Low-FODMAPs diet Trial
Antioxidants The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males 34071378
antioxidants TCA Cycle and Fatty Acids Oxidation Reflect Early Cardiorenal Damage in Normoalbuminuric Subjects with Controlled Hypertension 34356333
Association of plasma fatty acid-binding protein 3 with estimated glomerular filtration rate in patients with type 2 diabetes mellitus 34975301
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