Multiplex Assay Kit for Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay)

SERPINA1; SPAAT; A1-AT; PI; Serpin Peptidase Inhibitor,Clade A(Alpha-1 Antiproteinase/AntiTrypsin)Member 1; Alpha-1 protease inhibitor; Short peptide from AAT; Serpin A1

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 84-93 90
EDTA plasma(n=5) 78-89 84
heparin plasma(n=5) 80-95 86
sodium citrate plasma(n=5) 87-97 93

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 80-101% 89-97% 84-97%
EDTA plasma(n=5) 83-93% 80-101% 78-101% 98-105%
heparin plasma(n=5) 91-98% 80-92% 97-105% 87-95%
sodium citrate plasma(n=5) 92-105% 94-103% 94-101% 85-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:a1AT) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Biotechnology Expression and characterization of recombinant human alpha-antitrypsin in transgenic rice seed PubMed: 23376844
Sci Rep Hepatic steatosis depresses alpha-1-antitrypsin levels in human and rat acute pancreatitis PubMed: 26634430
PLoS One Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study PubMed: 25955845
Respir Care Diagnostic Utility of Biomarkers in COPD PubMed: 26106205
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach Pubmed:29315472
Journal of Clinical Medicine Urinary Proteomics for the Early Diagnosis of Diabetic Nephropathy in Taiwanese Patients Pubmed: 30486327
Journal of Proteomics Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection Pubmed: 31706945
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848
Pediatrics International Prenatal diagnosis of maternal serum from mothers carrying β‐thalassemic fetus 34559910
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPB697Hu01 Recombinant Alpha-1-Antitrypsin (a1AT) Positive Control; Immunogen; SDS-PAGE; WB.
PAB697Hu01 Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
LAB697Hu71 Biotin-Linked Polyclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC.
MAB697Hu24 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu25 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu23 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu29 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu27 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu26 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
MAB697Hu21 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC/IF.
MAB697Hu22 Monoclonal Antibody to Alpha-1-Antitrypsin (a1AT) WB; IHC; ICC; IP.
FAB697Hu02 Anti-Alpha-1-Antitrypsin (a1AT) Monoclonal Antibody Flow cytometry.
MEB697Hu Mini Samples ELISA Kit for Alpha-1-Antitrypsin (a1AT) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB697Hu ELISA Kit for Alpha-1-Antitrypsin (a1AT) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB697Hu CLIA Kit for Alpha-1-Antitrypsin (a1AT) Chemiluminescent immunoassay for Antigen Detection.
LMB697Hu Multiplex Assay Kit for Alpha-1-Antitrypsin (a1AT) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB697Hu01 ELISA Kit DIY Materials for Alpha-1-Antitrypsin (a1AT) Main materials for "Do It (ELISA Kit) Yourself".