Multiplex Assay Kit for Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay)

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-105 101
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 92-101 97
sodium citrate plasma(n=5) 81-92 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Salusin Alpha (SALa) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-105% 80-95% 94-105% 86-97%
EDTA plasma(n=5) 87-101% 87-104% 95-102% 79-94%
heparin plasma(n=5) 88-102% 93-105% 88-95% 95-102%
sodium citrate plasma(n=5) 95-102% 80-102% 88-102% 81-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SALa) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

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Magazine Citations
Peptides The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass PubMed: 22884920
Department of Medical Biochemistry and Clinical Biochemistry Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows ScienceDirect: S0196978113000533
European Journal of Obstetrics & Gynecology and Reproductive Biology Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. PubMed: 23178004
Biotechnic & Histochemistry Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome Pubmed: 23957705
Peptides Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus ScienceDirect: S0196978113003100
Med Sci Monit. Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome Pubmed:Pmc4136931
Pediatr Nephrol. Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? Pubmed:25245503
European Journal of Dermatology Serum salusin-α and salusin-β levels in patients with psoriasis PubMed: 26055619
Gynecol Endocrinol Serum salusins levels are increased and correlated positively with cyst size in ovarian endometrioma PubMed: 26008602
Multiple Sclerosis and Related Disorders Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis Pubmed: 30738876
cardiology in the young Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity Pubmed: 31486354
Drug and Chemical Toxicology Salusin-α mitigates diabetic nephropathy via inhibition of the Akt/mTORC1/p70S6K signaling pathway in diabetic rats Pubmed: 31665937
Journal of international medical research Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis Pubmed: 32054363
J Ultrasound Med Targeted Delivery of Salusin©\¦Á into Rabbit Carotid Arterial Endothelium Using SonoVue 33818784
Food Science & Nutrition Inhibition of oxidative stress and apoptosis by camel milk mitigates cyclosporine©\induced nephrotoxicity: Targeting Nrf2/HO©\1 and AKT/eNOS/NO pathways 34136182
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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