≡

Multiplex Assay Kit for Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay)

CMPD1; CMD1; SRA1; Campomelic Dysplasia,Autosomal Sex-Reversal; Transcription factor SOX-9

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-101 92
EDTA plasma(n=5) 78-101 88
heparin plasma(n=5) 80-94 89

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 88-96% 84-98% 78-95% 94-102%
EDTA plasma(n=5) 86-94% 99-105% 90-101% 91-101%
heparin plasma(n=5) 92-101% 79-93% 88-95% 88-96%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:SOX9) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
    add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Cell Biochem Biophys Next-Generation Sequencing of Circular RNAs Reveals the Molecular Mechanisms of Chondrogenic Differentiation in Human Adipose-derived Stem Cells Pubmed:35257277
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPG329Hu01 Recombinant Sex Determining Region Y Box Protein 9 (SOX9) Positive Control; Immunogen; SDS-PAGE; WB.
PAG329Hu01 Polyclonal Antibody to Sex Determining Region Y Box Protein 9 (SOX9) WB; IHC; ICC; IP.
MAG329Hu21 Monoclonal Antibody to Sex Determining Region Y Box Protein 9 (SOX9) WB; IHC; ICC; IP.
SEG329Hu ELISA Kit for Sex Determining Region Y Box Protein 9 (SOX9) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG329Hu Multiplex Assay Kit for Sex Determining Region Y Box Protein 9 (SOX9) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.