Mini Samples ELISA Kit for Interleukin 12 (IL12)


This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 12 (IL12).
No significant cross-reactivity or interference between Mini Samples Interleukin 12 (IL12) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Mini Samples Interleukin 12 (IL12) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Interleukin 12 (IL12) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 97-105 101
EDTA plasma(n=5) 88-101 91
heparin plasma(n=5) 97-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 12 (IL12) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 12 (IL12) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Interleukin 12 (IL12) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-94% 91-99% 96-104% 81-103%
EDTA plasma(n=5) 82-103% 80-99% 96-104% 79-103%
heparin plasma(n=5) 97-104% 81-93% 81-97% 92-99%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×4.5mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
Cancer Science A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme … Scholarena:Source
International Immunopharmacology Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment Pubmed:30015235
Oncology Letters Antitumor efficacy of the Runx2‑dendritic cell vaccine in triple‑negative breast cancer in vitro Pubmed:30127867
Neuroscience The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat joints Pubmed: 30248434
Cancer Biomarkers Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in … Pubmed: 30223387
International Journal of Clinical and Experimental Medicine The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
Experimental and Therapeutic Medicine RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery
Chemosphere Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus
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