Mini Samples ELISA Kit for Heat Shock Protein 70 (HSP70)
DnaK; 70 Kilodalton Heat Shock Proteins
- Product No.MEA873Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range3.12-200ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 1.19ng/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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This assay has high sensitivity and excellent specificity for detection of Mini Samples Heat Shock Protein 70 (HSP70).
No significant cross-reactivity or interference between Mini Samples Heat Shock Protein 70 (HSP70) and analogues was observed.
Matrices listed below were spiked with certain level of recombinant Mini Samples Heat Shock Protein 70 (HSP70) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Heat Shock Protein 70 (HSP70) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Heat Shock Protein 70 (HSP70) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Heat Shock Protein 70 (HSP70) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Heat Shock Protein 70 (HSP70) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×60µL||Assay Diluent A||1×6mL|
|Detection Reagent B||1×60µL||Assay Diluent B||1×6mL|
|TMB Substrate||1×4.5mL||Stop Solution||1×3mL|
|Wash Buffer (30 × concentrate)||1×10mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
|Journal of Molecular Medicine||Proteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy SpringerLink: b8738j3571287425|
|Journal of Neuroinflammation||Neuroinflammation in autism spectrum disorders PubMed: PMC3549857|
|Eur Arch Psychiatry Clin Neurosci.||Luteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder Pubmed: 23455589|
|Arch Bronconeumol||Concentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue PubMed: 26055465|
|Arch Bronconeumol||Heat Shock Proteins, PubMed: 26055465|
|Animal Science Journal||The effect of heat stress on gene expression and synthesis of heat‐shock and milk proteins in bovine mammary epithelial cells PubMed: 26467738|
|Transplant Proc||L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients PubMed: 26518955|
|Plos One||Long-Term Effects of Induced Hypothermia on Local and Systemic Inflammation-Results from a Porcine Long-Term Trauma Model Pubmed:27144532|
|J Pak Med Assoc.||Elevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine mnstemps:33-1429777310|
|Egyptian Poultry Science Journal||STUDY THE RELATIONSHIP BETWEEN SELENIUM AND HEAT SHOCK PROTEINS UNDER HEAT STRESS FOR LOCAL SINAI CHICKENS STRAIN abstract:3d115259031|
|Physiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load S0144860916301935|
|Animal Science Journal||Prokaryotic expression of chicken interferon-γ fusion protein and its effect on expression ofpoultry heat shock protein 70 under heat stress. pubmed:27753175|
|Pharmacol Rep||High-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver. pubmed:28531762|
|Biomed Pharmacother.||SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. pubmed:28122311|
|Immunobiology||Concentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer pubmed:29111316|
|Egyptian Poultry Science Journal||EFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS. 125788660|
|Biomedicine & Pharmacotherapy||SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death DOI:10.1016/j.biopha.2017.01.087|
|Egyptian Poultry Science Journal||IMPACT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON PRODUCTIVE PERFORMANCE, IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF BROILER CHICKENS UNDER HEAT-STRESS CONDITION. ISSN: 1110-5623|
|Aquaculture||Physiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems 10.1016/j.aquaculture.2017.11.030|
|Journal of dairy research||Detection of 70 kDa heat shock protein in the saliva of dairy cows. pubmed:28625184|
|Journal of animal science||Reducing rumen starch fermentation of wheat with three percent sodium hydroxide has the potential to ameliorate the effect of heat stress in grain-fed wethers pubmed:29293755|
|Biochemistry and Cell Biology||Ectopic expression of Miro 1 ameliorates seizures and inhibits hippocampal neurodegeneration in a mouse model of pilocarpine epilepsy Pubmed:29365285|
|Egyptian Poultry Science Journal||THE EFFECT OF CHROMIUM PICOLINATE SUPPLEMENTATION ON IMMUNE RESPONSE AND HEAT SHOCK PROTEINS OF LAYING HENS UNDER HEAT … article_5570_5c06907ac09a7724a58d2b796fe91aba.pdf|
|Egyptian Poultry Science Journal||EFFECT OF MITIGATION OF HEAT STRESS BY EARLY HEATE ACCLIMATION AND GLUTAMINE INJECTION ON SOME PHYSIOLOGICAL MEASUREMENTS … :|
|FASEB Journal||Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure Pubmed: 30230922|
|Journal of Biological Sciences||Maged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker|
|Biological Procedures Online||Effective Visualization and Easy Tracking of Extracellular Vesicles in Glioma Cells|
|Journal of Biological Sciences||Protective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer|
|博士论文||PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
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