Mini Samples ELISA Kit for Cholecystokinin 8 (CCK8)

Sincalide; Cholecystokinin 8, Octapeptide

Specificity

This assay has high sensitivity and excellent specificity for detection of Mini Samples Cholecystokinin 8 (CCK8).
No significant cross-reactivity or interference between Mini Samples Cholecystokinin 8 (CCK8) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Mini Samples Cholecystokinin 8 (CCK8) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Cholecystokinin 8 (CCK8) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-94 90
EDTA plasma(n=5) 81-102 97
heparin plasma(n=5) 82-99 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Cholecystokinin 8 (CCK8) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 96-105% 81-95% 99-105%
EDTA plasma(n=5) 78-101% 85-93% 94-101% 80-95%
heparin plasma(n=5) 94-103% 90-101% 89-96% 93-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×60µL Assay Diluent A 1×6mL
Detection Reagent B 1×60µL Assay Diluent B 1×6mL
TMB Substrate 1×9mL Stop Solution 1×3mL
Wash Buffer (30 × concentrate) 1×10mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well.
    And then add 25μL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 50µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 50µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 25µL Stop Solution. Read at 450 nm immediately.

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Magazine Citations
World J Gastroenterol Roles of sphincter of Oddi motility and serum vasoactive intestinal peptide, gastrin and cholecystokinin octapeptide NCBI: PMC4000510
Epilepsy Research A prospective study on changes in blood levels of cholecystokinin-8 and leptin in patients with refractory epilepsy treated with the ketogenic diet. pubmed:27568597
Journal of Ethnopharmacology Luhong formula inhibits myocardial fibrosis in a paracrine manner by activating thegp130/JAK2/STAT3 pathway in cardiomyocytes. pubmed:28115285
Untersuchungen zu verschiedenen biochemischen Markern des zentralen Nervensystems im Liquor cerebrospinalis von Patienten mit der Huntington-Krankheit :
Journal of Functional Foods The protective effect of Capparis spinosa fruit on triptolide-induced acute liver injury: A metabolomics-based systematic study
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
CPB044Hu21 OVA Conjugated Cholecystokinin 8 (CCK8) Immunogen; SDS-PAGE; WB.
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PAB044Hu01 Polyclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
LAB044Hu71 Biotin-Linked Polyclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC.
MAB044Hu21 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu22 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu23 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu24 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu25 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu27 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MAB044Hu28 Monoclonal Antibody to Cholecystokinin 8 (CCK8) WB; IHC; ICC; IP.
MEB044Hu Mini Samples ELISA Kit for Cholecystokinin 8 (CCK8) Enzyme-linked immunosorbent assay for Antigen Detection.
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LMB044Hu Multiplex Assay Kit for Cholecystokinin 8 (CCK8) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.