Polyclonal Antibody to Collagen Type IV (COL4)

Type-IV Collagen

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against COL4. It has been selected for its ability to recognize COL4 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Nature Medicine Angiotensin-Converting Enzyme 2 Overexpression Remarkably Ameliorated Glomerular Injury in a Rat Model of Diabetic Nephropathy: A Comparison with ACE Inhibition MolMed: 10_11_liu
American Journal of Physiology - Renal Physiology Advanced oxidation protein products induce mesangial cell perturbation through PKC-dependent activation of NADPH oxidase PubMed: 19019916
Journal of Surgical Research Serum Levels of Matrix Metalloproteinase-2 as a Marker of Intimal Hyperplasia ScienceDirect: S0022480409001942
Stem Cell Research Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly Pubmed: 24739658
Molecules. Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells. Pubmed: 24013407
Journal of Ethnopharmacology Bioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathy ScienceDirect: S0378874114002414
Journal of Clinical and Experimental Hepatology Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis ScienceDirect: S0973688314000073
Life Sci. Attenuation of renal ischemia/reperfusion injury by a?aí extract preconditioning in a rat model Pubmed:25476829
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media Pubmed:Pmc4130592
American Journal fo Reproductive Immunology Trophoblasts and Decidual Stromal Cells Regulate Decidual NK Cell Functions Via Interaction between Collagen and LAIR-1 Pubmed:24548186
Chem Biol Interact Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis PubMed: 25617480
Viral Hepatitis Journal The Relationship of the Degree of Hepatic Fibrosis with Hyaluronic Acid, Type 4 Collagen, and Procollagen Type 3 N-Terminal Peptide Levels in Patients with Chronic Viral Hepatitis Openview: 8A0B8C97D72C61741371F33508760B75
International Journal of Experimental Pathology Pathological characterization and morphometric analysis of hepatic lesions in SHRSP5/Dmcr, an experimental non-alcoholic steatohepatitis model, induced by high-fat and high-cholesterol diet doi:10.1111
MOLECULAR AND CLINICAL ONCOLOGY Clinical significance of serum laminin and type‑IV collagen levels in cutaneous melanoma patients Pubmed:27330797
Am J Transplant. Key Matrix Proteins Within the Pancreatic Islet Basement Membrane Are Differentially Digested During Human Islet Isolation. pubmed:27456745
Molecular Medicine Reports Human decidua mesenchymal stem cells regulate decidual natural killer cell function via interactions between collagen and leukocyte‑associated immunoglobulin‑like receptor 1 10.3892:mmr.2017.6921
Clinical Cancer Research Phase I and biomarker study of plerixafor and bevacizumab in recurrent high-grade glioma Pubmed:29941486
International Journal of Molecular Medicine Adenovirus‑mediated knockdown of activin A receptor type 2A attenuates immune‑induced hepatic fibrosis in mice and inhibits interleukin‑17‑induced … Pubmed:29620144
Biomedicine & Pharmacotherapy Corn silk (Zea mays L.), a source of natural antioxidants with α-amylase, α-glucosidase, advanced glycation and diabetic nephropathy inhibitory activities Pubmed: 30530231
Experimental Lung Research High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects Pubmed: 31290711
Journal of Biological Chemistry Growth hormone induces Notch1 signaling in podocytes and contributes to proteinuria in diabetic nephropathy Pubmed: 31511328
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Pantoea agglomerans chronic exposure induces epithelial-mesenchymal transition in human lung epithelial cells and mice lungs Pubmed: 32146192
PLoS One Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment¡ªIn vivo studies 33999928
Advanced glycation end-products associate with podocytopathy in type II diabetic patients
Biomolecules Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study Pubmed:35625637
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
NPA180Hu01 Native Collagen Type IV (COL4) Positive Control; Immunogen; SDS-PAGE; WB.
PAA180Hu01 Polyclonal Antibody to Collagen Type IV (COL4) WB; IHC; ICC; IP.
LAA180Hu71 Biotin-Linked Polyclonal Antibody to Collagen Type IV (COL4) WB; IHC; ICC.
SEA180Hu ELISA Kit for Collagen Type IV (COL4) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA180Hu High Sensitive ELISA Kit for Collagen Type IV (COL4) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA180Hu CLIA Kit for Collagen Type IV (COL4) Chemiluminescent immunoassay for Antigen Detection.
LMA180Hu Multiplex Assay Kit for Collagen Type IV (COL4) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA180Hu01 ELISA Kit DIY Materials for Collagen Type IV (COL4) Main materials for "Do It (ELISA Kit) Yourself".