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Multiplex Assay Kit for Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array)
PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein
- Product No.CMA114Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Sample TypeSerum, plasma, tissue homogenates and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length4h
- Detection Range0.4883-500pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.1628pg/mL.
- DownloadInstruction Manual
- UOM 48T96T
- FOB
US$ 419
US$ 598
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array).
No significant cross-reactivity or interference between Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) and the recovery rates were calculated by comparing the measured value to the expected amount of Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-105 | 101 |
| EDTA plasma(n=5) | 80-88 | 81 |
| heparin plasma(n=5) | 78-102 | 91 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Placenta Growth Factor (PLGF) ,etc. by CBA (Cytometric Bead Array) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 85-101% | 86-94% | 86-95% | 83-95% |
| EDTA plasma(n=5) | 78-90% | 80-102% | 88-95% | 98-105% |
| heparin plasma(n=5) | 97-105% | 96-104% | 94-102% | 78-101% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 100μL standard or sample to each well, add 10μL magnetic beads, and incubate 120 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60 minutes protect from light at 25°C on shaker;
5. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
6. Wash plate once on magnetic frame;
7. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
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| Magazine | Citations |
| Microvascular Research | Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289 |
| Oncogene | Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069 |
| ?eLife | Photoreceptor avascular privilege is shielded by soluble VEGF receptor-1 PubMed: PMC3687373 |
| International Journal of Nanomedicine | Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis Pubmed:27042064 |
| The Journal of Maternal-Fetal & Neonatal Medicine | Serum angiogenic profile in abnormal placentation Pubmed:26863111 |
| Fertility and Sterility | Administration of atosiban in patients with endometriosis undergoing frozenâthawed embryo transfer: a prospective, randomized study Pubmed:27143518 |
| Basic & Clinical Pharmacology & Toxicology | Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway doi:10.1111 |
| Life Sci. | Placenta growth factor contributes to cell apoptosis and epithelial-to-mesenchymal transition in the hyperoxia-induced acute lung injury Pubmed:27211521 |
| Int Immunopharmacol | Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway Pubmed:27280587 |
| Journal of Maternal-Fetal & Neonatal Medicine | Relationship between elevated serum level of placental growth factor and status of gestational diabetes mellitus Pubmed: 30935303 |
| Children (Basel) | sFlt-1/PlGF Ratio in Prediction of Short-Term Neonatal Outcome of Small for Gestational Age Neonates 34438609 |
| Dose-Response: An International Journal | Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth Factor Pubmed:35110976 |