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Active Placenta Growth Factor (PLGF)

PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein

ACTIVITY TEST

A114Mu is a research-grade monoclonal antibody specifically targeting Placenta Growth Factor (PLGF). Monoclonal antibodies like A114Mu are laboratory-engineered proteins designed to bind to a single, precise epitope on their target molecule—here, PLGF. Its development stems from the need to study PLGF’s biological roles, particularly in angiogenesis (blood vessel formation) and pathological conditions such as cancer and preeclampsia. A114Mu enables researchers to block PLGF activity in vitro and in vivo, helping elucidate its interactions with receptors like VEGFR-1. This tool is critical for validating PLGF as a therapeutic target, as its inhibition may suppress abnormal blood vessel growth in tumors or regulate placental vascular development in pregnancy-related disorders.To detect the activity of recombinant PLGF, a functional ELISA assay was performed to evaluate the interaction between recombinant human PLGF and recombinant human NRP1.Briefly, biotin-linked PLGF were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100µl were then transferred to NRP1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µl stop solution to the wells and read at 450nm immediately. The binding activity of PLGF and NRP1 was shown in Figure 1, the EC50 for this effect is 1.12355µg/mL.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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INCREMENT SERVICES

Magazine Citations
Microvascular Research Mutant hypoxia inducible factor-1α improves angiogenesis and tissue perfusion in ischemic rabbit skeletal muscle PubMed: 20937289
Oncogene Elevated PLGF contributes to small-cell lung cancer brain metastasis PubMed: 22797069
?eLife Photoreceptor avascular privilege is shielded by soluble VEGF receptor-1 PubMed: PMC3687373
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis Pubmed:27042064
The Journal of Maternal-Fetal & Neonatal Medicine Serum angiogenic profile in abnormal placentation Pubmed:26863111
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study Pubmed:27143518
Basic & Clinical Pharmacology & Toxicology Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway doi:10.1111
Life Sci. Placenta growth factor contributes to cell apoptosis and epithelial-to-mesenchymal transition in the hyperoxia-induced acute lung injury Pubmed:27211521
Int Immunopharmacol Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway Pubmed:27280587
Journal of Maternal-Fetal & Neonatal Medicine Relationship between elevated serum level of placental growth factor and status of gestational diabetes mellitus Pubmed: 30935303
Children (Basel) sFlt-1/PlGF Ratio in Prediction of Short-Term Neonatal Outcome of Small for Gestational Age Neonates 34438609
Dose-Response: An International Journal Puerarin Reduces Radiation-Induced Vascular Endothelial Cell Damage Via miR-34a/Placental Growth Factor Pubmed:35110976
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPA114Mu01 Recombinant Placenta Growth Factor (PLGF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA114Mu02 Recombinant Placenta Growth Factor (PLGF) Positive Control; Immunogen; SDS-PAGE; WB.
APA114Mu61 Active Placenta Growth Factor (PLGF) Cell culture; Activity Assays.
EPA114Mu61 Eukaryotic Placenta Growth Factor (PLGF) Positive Control; Immunogen; SDS-PAGE; WB.
PAA114Mu01 Polyclonal Antibody to Placenta Growth Factor (PLGF) WB
PAA114Mu02 Polyclonal Antibody to Placenta Growth Factor (PLGF) WB; IHC; ICC; IP.
LAA114Mu81 FITC-Linked Polyclonal Antibody to Placenta Growth Factor (PLGF) WB; IHC; ICC; IF.
LAA114Mu71 Biotin-Linked Polyclonal Antibody to Placenta Growth Factor (PLGF) WB; IHC; ICC.
SEA114Mu ELISA Kit for Placenta Growth Factor (PLGF) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA114Mu Multiplex Assay Kit for Placenta Growth Factor (PLGF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.