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ELISA Kit DIY Materials for Complement Factor H (CFH)

CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein

  • Product No.KSA635Ra11
  • Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
  • Reagent Contents Capture Antibody, Biotin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
  • Detectable SampleSerum, plasma and other biological fluids.
  • Applicable PrincipleCompetitive Inhibition ELISA for Antigen Detection
  • Detectable Range12.35-1000ng/mL
  • Applicable Sensitivity5.77ng/mL
  • ApplicationsMain materials for "Do It (ELISA Kit) Yourself".
  • Downloadn/a
  • UOM 96T*596T*10 96T*20 96T*50 96T*100
  • FOB US$ 1231  US$ 2052  US$ 3694  US$ 7182  US$ 12312 
    For more details, please contact local distributors!

SPECIFITY

The Abs in the kit have high sensitivity and excellent specificity for detection of Complement Factor H (CFH). No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.

USAGE

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

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Magazine Citations
Pediatr Int Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527
Hernia Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia Pubmed:29484522
Mikrochim Acta Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H Pubmed: 31187271
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H 33788378
Front Immunol A Family Affair: Addressing the Challenges of Factor H and the Related Proteins 33868311
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KSA635Ra11 ELISA Kit DIY Materials for Complement Factor H (CFH) Main materials for "Do It (ELISA Kit) Yourself".