Packages (Simulation)
Reagent Preparation
Image (I)
-
Results demonstration
Image (II)
-
Typical Standard Curve
Quality Guarantee
Instruction Manual
Certificate
Multiplex Assay Kit for Complement Factor H (CFH) ,etc. by FLIA (Flow Luminescence Immunoassay)
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
(Note: Up to 8-plex in one testing reaction)
- Product No.LMA635Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Sample TypeSerum, plasma and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length3h
- Detection Range0.98-1000ng/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 0.327 ng/mL.
- DownloadInstruction Manual
- UOM 8Plex 7Plex 6Plex 5Plex 4Plex 3Plex 2Plex1Plex
- FOB
US$ 427
US$ 443
US$ 468
US$ 501
US$ 534
US$ 583
US$ 657
US$ 821
Add to Price Calculator
Result
For more details, please contact local distributors!
Specificity
This assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH).
No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Complement Factor H (CFH) and the recovery rates were calculated by comparing the measured value to the expected amount of Complement Factor H (CFH) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-104 | 97 |
| EDTA plasma(n=5) | 86-96 | 91 |
| heparin plasma(n=5) | 78-105 | 98 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Complement Factor H (CFH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 95-102% | 84-96% | 94-105% |
| EDTA plasma(n=5) | 92-105% | 81-89% | 97-104% | 95-103% |
| heparin plasma(n=5) | 80-90% | 96-105% | 86-95% | 79-94% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:CFH) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Add 200μL analysis buffer solution to each well of the plate for pre-wetting;
2. Preparation of standards, reagents and samples before the experiment;
3. Add 50μL standard or sample to each well, add 50μL prepared Detection Reagent A, add 10μL magnetic beads, and incubate 90 minutes protect from light at 25°C on shaker;
4. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent B, and incubate 30 minutes protect from light at 25°C on shaker;
5. Wash plate once on magnetic frame;
6. Add 100μL sheath fluid, swirl for 10 minutes, read on the machine.
GIVEAWAYS
INCREMENT SERVICES
| Magazine | Citations |
| Pediatr Int | Atypical hemolytic uremic syndrome: Korean pediatric series PubMed: 25443527 |
| Hernia | Circulating matrix metalloproteinases and procollagen propeptides in inguinal hernia Pubmed:29484522 |
| Mikrochim Acta | Fluorescent silver nanoclusters as antibody label in a competitive immunoassay for the complement factor H Pubmed: 31187271 |
| Diagnostics | Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J Pubmed: 32585848 |
| JOURNAL OF CELLULAR AND MOLECULAR MEDICINE | The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H 33788378 |
| Front Immunol | A Family Affair: Addressing the Challenges of Factor H and the Related Proteins 33868311 |