Simplex Assay Kit for Dehydroepiandrosterone (DHEA) ,etc.

(Note: Up to 8-plex in one testing reaction)

Specificity

This assay has high sensitivity and excellent specificity for detection of Dehydroepiandrosterone (DHEA) ,etc..
No significant cross-reactivity or interference between Dehydroepiandrosterone (DHEA) ,etc. and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Dehydroepiandrosterone (DHEA) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Dehydroepiandrosterone (DHEA) ,etc. in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 96
EDTA plasma(n=5) 88-96 91
heparin plasma(n=5) 85-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Dehydroepiandrosterone (DHEA) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Dehydroepiandrosterone (DHEA) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Dehydroepiandrosterone (DHEA) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 81-101% 93-101% 92-103%
EDTA plasma(n=5) 89-102% 97-105% 95-105% 96-105%
heparin plasma(n=5) 93-101% 91-105% 92-101% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:DHEA) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
journal of molecular neuroscience Possible Metabolic Alterations among Autistic Male Children: Clinical and Biochemical Approaches Pubmed: 30600432
Ecotoxicol Environ Safe A preliminary study on the mechanism of the neurosteroid-mediated ionotropic receptor dysfunction in neurodevelopmental toxicity induced by?¡­ 33862428
Bioengineered MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1 34637688
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CPA398Ge11 BSA Conjugated Dehydroepiandrosterone (DHEA) Immunogen; SDS-PAGE; WB.
CPA398Ge21 OVA Conjugated Dehydroepiandrosterone (DHEA) Immunogen; SDS-PAGE; WB.
PAA398Ge01 Polyclonal Antibody to Dehydroepiandrosterone (DHEA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA398Ge28 Monoclonal Antibody to Dehydroepiandrosterone (DHEA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA398Ge21 Monoclonal Antibody to Dehydroepiandrosterone (DHEA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA398Ge29 Monoclonal Antibody to Dehydroepiandrosterone (DHEA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
RAA398Ge21 Recombinant Antibody to Dehydroepiandrosterone (DHEA) WB; IFC; IHC; IP.
MAA398Ge22 Monoclonal Antibody to Dehydroepiandrosterone (DHEA) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA398Ge ELISA Kit for Dehydroepiandrosterone (DHEA) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA398Ge Instant ELISA Kit for Dehydroepiandrosterone (DHEA) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA398Ge CLIA Kit for Dehydroepiandrosterone (DHEA) Chemiluminescent immunoassay for Antigen Detection.
LMA398Ge Simplex Assay Kit for Dehydroepiandrosterone (DHEA) ,etc. Simplex Assay for Antigen Detection.
KSA398Ge11 ELISA Kit DIY Materials for Dehydroepiandrosterone (DHEA) Main materials for "Do It(ELISA Kit) Yourself"