Multiplex Assay Kit for 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay)

8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG

(Note: Up to 8-plex in one testing reaction)


This assay has high sensitivity and excellent specificity for detection of 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay).
No significant cross-reactivity or interference between 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) and analogues was observed.


Matrices listed below were spiked with certain level of 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) and the recovery rates were calculated by comparing the measured value to the expected amount of 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-101 95
EDTA plasma(n=5) 78-98 89
heparin plasma(n=5) 87-101 91
sodium citrate plasma(n=5) 86-104 95


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 91-105% 89-98% 79-95%
EDTA plasma(n=5) 92-105% 81-91% 87-95% 82-97%
heparin plasma(n=5) 86-101% 79-99% 84-95% 91-98%
sodium citrate plasma(n=5) 80-102% 81-104% 91-99% 89-96%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
96-well plate 1 Plate sealer for 96 wells 4
Pre-Mixed Standard 2 Standard Diluent 1×20mL
Pre-Mixed Magnetic beads (22#:8-OHdG) 1 Analysis buffer 1×20mL
Pre-Mixed Detection Reagent A 1×120μL Assay Diluent A 1×12mL
Detection Reagent B (PE-SA) 1×120μL Assay Diluent B 1×12mL
Sheath Fluid 1×10mL Wash Buffer (30 × concentrate) 1×20mL
Instruction manual 1

Assay procedure summary

1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
    add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.



Magazine Citations
Yonsei Med J. Iodinated contrast media can induce long-lasting oxidative stress in hemodialysis patients. Pubmed: 24142649
J Histochem Cytochem Glycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in Rats PubMed: 26385569
Diabetes/Metabolism Research and Reviews Reduced peripheral blood mtDNA content is associated with impaired glucose‐stimulated islet β cell function in a Chinese population with different degrees of … doi:10.1002
Nutrition Journal Influence of diet on leukocyte telomere length, markers of inflammation and oxidative stress in individuals with varied glucose tolerance: a Chinese population study Pubmed:27071648
Diabetology & Metabolic Syndrome Oxidative DNA damage and oxidized low density lipoprotein in Type II diabetes mellitus among patients with Helicobacter pylori infection pmc:PMC4855822
UVaDOC Dieta hipercalórica e hipoxia intermitente crónica como modelos de obesidad y apnea obstructiva del sueño. Significado de la leptina en el control respiratorio Tesis1149-161108.pdf
Journal of Diabetes Mellitus The Activity of Serum 8-Iso-Prostaglandin F2α as Oxidative Stress Marker in Patients with Diabetes Mellitus Type 2 and Associated Dyslipidemic Hyperglycemia profile:Wesam_Nasef3
Indian Journal of Hematology and Blood Transfusion Oleuropein Ameliorates Cisplatin-induced Hematological Damages Via Restraining Oxidative Stress and DNA Injury article:10.1007
Journal of Diabetes Research Oxidative Damage to the Salivary Glands of Rats with Streptozotocin-Induced Diabetes-TemporalStudy: Oxidative Stress and Diabetic Salivary Glands. pubmed:27478848
Front Physiol. Oxidative Modification in the Salivary Glands of High Fat-Diet Induced Insulin Resistant Rats. pubmed:28184199
Archives of Oral Biology Chronic high-protein diet induces oxidative stress and alters the salivary gland function in rats pubmed:28926744
BioMed Research International Oxidative modification of biomolecules in the non-stimulated and stimulated saliva of patients with morbid obesity treated with bariatric surgery 10.1155:2017
Oncotarget Chemopreventive effects of atractylenolide II on mammary tumorigenesis via activating Nrf2-ARE pathway pubmed:29100404
International Journal of Molecular Sciences Antioxidant Defence, Oxidative Stress and Oxidative Damage in Saliva, Plasma and Erythrocytes of Dementia Patients. Can Salivary AGE be a Marker of Dementia? pubmed:29053628
Antioxidants and Redox Signaling Chemokine Receptor CXCR4 Plays a Crucial Role in Mediating Oxidative StressInduced Podocyte Injury 10.1089:ars.2016.6758
Melatonin ameliorates oxidative DNA damage and protects against formaldehyde-induced oxidative stress in rats ISSN:1940-5901/IJCEM0048822
Biological Trace Element Research Protective Effect of Origanum Oil on Alterations of Some Trace Elements and Antioxidant Levels Induced by Mercuric Chloride in Male Rats pubmed:28616770
HAYATI Journal of Biosciences Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult SpragueeDawley Rats 10.1016/j.hjb.2017.08.007
Scientific Reports Risk of glomerular filtration rate decline in patients with hypertrophic cardiomyopathy and obstructive sleep apnoea pubmed:29234143
Journal of Pain Research Sex differences in complex regional pain syndrome type I (CRPS-I) in mice pubmed:28831269
HAYATI Journal of Biosciences Micronuclei Formation and 8-Hydroxy-2-Deoxyguanosine Enzyme Detection in Ovarian Tissues After Radiofrequency Exposure at 1800 MHz in Adult Sprague–Dawley Rats 10.1016/j.hjb.2017.08.007
Oxidative Medicine and Cellular Longevity Effect of N-Acetylcysteine on Antioxidant Defense, Oxidative Modification, and Salivary Gland Function in a Rat Model of Insulin Resistance Pubmed:29636851
Oxidative medicine and cellular longevity Exposure to Ti4Al4V Titanium Alloy Leads to Redox Abnormalities, Oxidative Stress, and Oxidative Damage in Patients Treated for Mandible Fractures Pubmed:30013720
Journal of Cardiovascular Development and Disease Oxidative DNA Damage and Carotid Intima Media Thickness as Predictors of Cardiovascular Disease in Prediabetic Subjects Pubmed:29518984
Reproductive Biology Anti-hypercholesterolemic impacts of barley and date palm fruits on the ovary of Wistar albino rats and their offspring Pubmed:30005909
Oxidative medicine and cellular longevity Redox Balance, Antioxidant Defense, and Oxidative Damage in the Hypothalamus and Cerebral Cortex of Rats with High Fat Diet-Induced Insulin Resistance
Journal of Pharmacological Reports Journal of Pharmacological Reports Pubmed: 27489887
Diabetes & Metabolic Syndrome: Clinical Research & Reviews Cross-sectional correlates of oxidative stress and inflammation with glucose intolerance in prediabetes
BioMed Research International Oral Administration of Lactobacillus delbrueckii during the Suckling Phase Improves Antioxidant Activities and Immune Responses after the Weaning Event in a Piglet …
Oxidative Medicine and Cellular Longevity Luteolin Attenuates Cardiac Ischemia/Reperfusion Injury in Diabetic Rats by Modulating Nrf2 Antioxidative Function
Oxidative Medicine and Cellular Longevity Dendrobium Officinale Polysaccharides Protect against MNNG-Induced PLGC in Rats via Activating the NRF2 and Antioxidant Enzymes HO-1 and NQO-1
International Journal of Pharmaceutical Research & Allied Sciences Prophylactic Impact of Beta vulgaris L in Ameliorating Cyclosporine A-Induced Hepatotoxicity in Rats.
Journal of Diabetes and Metabolism Assessment of 8-Hydroxy-2-Deoxyguanosine (8-OHdG) as a Diagnostic Marker for Diabetic Nephropathy in Type 1 Egyptian Diabetic Patients
Journal of Food Biochemistry Dietary supplementation of barley and/or dates attenuate hypercholesterolemic‐induced endometrial dysfunction in Wistar albino rats via alleviation of apoptotic … Pubmed: 31373029
environmental science and pollution research Possible prophylactic effect of omega-3 fatty acids on cadmium-induced neurotoxicity in rats' brains Pubmed: 31468353
Oxidative Medicine and Cellular Longevity Leukocyte Telomere Length Independently Predicts 3-Year Diabetes Risk in a Longitudinal Study of Chinese Population Pubmed: 32215181
Journal of Clinical Medicine Dysfunction of Salivary Glands, Disturbances in Salivary Antioxidants and Increased Oxidative Damage in Saliva of Overweight and Obese Adolescents Pubmed: 32079369
Frontiers in Pharmacology Hydrogen Sulfide Attenuates Particulate Matter-Induced Emphysema and Airway Inflammation Through Nrf2-Dependent Manner Pubmed: 32116706
Odontology Salivary 8-hydroxyguanosine levels in smokers and non-smokers with chronic periodontitis Pubmed: 32065311
FREE RADICAL RESEARCH Negative association between antioxidant vitamin intake and non-alcoholic fatty liver disease in Chinese non-diabetic adults: mediation models involving superoxide … Pubmed: 32985285
JOURNAL OF FOOD SCIENCE Lycopene protects neuroblastoma cells against oxidative damage via depression of ER stress Pubmed: 32885410
Medicine Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study Pubmed: 32756181
Lipids Health Dis Triglyceride is independently correlated with insulin resistance and islet beta cell function: a study in population with different glucose and lipid metabolism … Pubmed: 32487177
Theranostics Rescuing Dicer expression in inflamed colon tissues alleviates colitis and prevents colitis-associated tumorigenesis Pubmed: 32483416
Journal of Clinical Medicine Systemic Redox Imbalance in Patients with Chronic Granulomatous Disease Pubmed: 32397350
Oxidative Medicine and Cellular Longevity NAC Supplementation of Hyperglycemic Rats Prevents the Development of Insulin Resistance and Improves Antioxidant Status but Only Alleviates General … Pubmed: 33274008
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY Potential Protective Effect of Dietary Intake of Non-α-Tocopherols on Cellular Aging Markers Mediated by Tumor Necrosis Factor-α in Prediabetes: A Cross … Pubmed: 32509152
oxidative medine cellular longevity Relationship between Decreased Serum Superoxide Dismutase Activity and Metabolic Syndrome: Synergistic Mediating Role of Insulin Resistance and β … Pubmed: 32617139
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS Induction of genotoxicity and mutagenic potential of heavy metals in Thai occupational workers Pubmed: 32928371
Journal of Functional Foods Oral administration of Lactobacillus delbrueckii during the suckling period improves intestinal integrity after weaning in piglets
Journal of Diabetes Research Changes and Risk Factors of Skeletal Muscle Mass and Strength in Patients with Type 2 Diabetes over 60 Years Old: A Cross-Sectional Study from China
The potential protective role of gamma-irradiated vaccine versus Punica granatum treatment against murine trichinellosis
Mutat Res Gen Tox En
Electroacupuncture ameliorates motor dysfunction via inhibiting p66Shc-mediated oxidative stress and endoplasmic reticulum stress in rats with spinal cord …
Journal of Skin and Stem Cell 8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment
Redox Biol Biomarkers of nucleic acid oxidation¨Ca summary state-of-the-art 33579665
Theranostics Antioxidant activity of mesenchymal stem cell-derived extracellular vesicles restores hippocampal neurons following seizure damage 33897894
Exp Ther Med Naringenin alleviates myocardial ischemia reperfusion injury by enhancing the myocardial miR-126-PI3K/AKT axis in streptozotocin-induced diabetic rats 34093766
chemosphere Oxidative stress-and mitochondrial dysfunction-mediated cytotoxicity by silica nanoparticle in lung epithelial cells from metabolomic perspective 33662726
Food Funct. Sestrin2 is involved in Nrf2-regulated antioxidative signaling pathway in luteolin-induced prevention of the diabetic rat heart from ischemia/reperfusion injury 33900303
Aquaculture Reports Growth performance, antioxidant capacity, tissue fatty acid composition and lipid metabolism of juvenile green mud crab Scylla paramamosain in response to?¡­
Ann Behav Med Role of Yoga and Its Plausible Mechanism in the Mitigation of DNA Damage in Type-2 Diabetes: A Randomized Clinical Trial 34180500
Cancer Manag Res Association of Tumour Microenvironment with Protein Glycooxidation, DNA Damage, and Nitrosative Stress in Colorectal Cancer 34408493
The Journal of Clinical Endocrinology & Metabolism High Hemoglobin glycation index is associated with telomere attrition independent of HbA1c, mediating by TNFα 34562085
Ecotoxicol Environ Saf Chromium induced neurotoxicity by altering metabolism in zebrafish larvae 34781135
Toxins Evaluation of the Therapeutic Effects of Protocatechuic Aldehyde in Diabetic Nephropathy 34437430
J Int Med Res Body mass index is associated with Helicobacter pylori infection and increased oxidative DNA damage in an obese population Pubmed:35209724
Ann Transplant Biochemical and Molecular Studies of Different Parameters as Markers for Nephropathy in Type 1 Diabetic Patients Pubmed:34751188
Oxidative Medicine and Cellular Longevity Blockage of Fibronectin 1 Ameliorates Myocardial Ischemia/Reperfusion Injury in Association with Activation of AMP-LKB1-AMPK Signaling Pathway Pubmed:35602095
Catalog No. Related products for research use of Pan-species (General) Organism species Applications (RESEARCH USE ONLY!)
CEA660Ge ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA660Ge CLIA Kit for 8-Hydroxydeoxyguanosine (8-OHdG) Chemiluminescent immunoassay for Antigen Detection.
LMA660Ge Multiplex Assay Kit for 8-Hydroxydeoxyguanosine (8-OHdG) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.