CLIA Kit for Eosinophil Chemotactic Factor (ECF)

CCL11; SCYA11; Eotaxin 1; Eosinophil Chemotactic Protein 1; Chemokine C-C-Motif Ligand 11; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 11


This assay has high sensitivity and excellent specificity for detection of Eosinophil Chemotactic Factor (ECF).
No significant cross-reactivity or interference between Eosinophil Chemotactic Factor (ECF) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Eosinophil Chemotactic Factor (ECF) and the recovery rates were calculated by comparing the measured value to the expected amount of Eosinophil Chemotactic Factor (ECF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 80-91 88
heparin plasma(n=5) 94-101 97


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Eosinophil Chemotactic Factor (ECF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Eosinophil Chemotactic Factor (ECF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 86-104% 78-102% 98-105%
EDTA plasma(n=5) 83-101% 87-95% 85-93% 96-104%
heparin plasma(n=5) 95-103% 79-99% 84-101% 87-99%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.



Magazine Citations
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY Elevated Serum Level of CD48 in Patients with Intermittent Allergic Rhinitis Pubmed: 32966985
Exp Mol Pathol The regulatory role of SFRP5/WNT5A axis in allergic rhinitis through inhibiting JNK pathway activation and lowering mucin generation in human nasal?¡­ 33285209
Immunopharmacol Immunotoxicol sCD48 is elevated in non-allergic but not in allergic persistent rhinitis 34477021
Exp Eye Res Sulforaphane inhibits cytokine-stimulated chemokine and adhesion molecule expressions in human corneal fibroblasts: Involvement of the MAPK, STAT, and NF-κB … Pubmed:35038457
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