ELISA Kit for Alpha-Fetoprotein (AFP)

aFP; A-FP; FETA; HPAFP; Alpha-Fetoglobulin; Alpha-1-fetoprotein


This assay has high sensitivity and excellent specificity for detection of Alpha-Fetoprotein (AFP).
No significant cross-reactivity or interference between Alpha-Fetoprotein (AFP) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Alpha-Fetoprotein (AFP) and the recovery rates were calculated by comparing the measured value to the expected amount of Alpha-Fetoprotein (AFP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-89 85
EDTA plasma(n=5) 81-101 92
heparin plasma(n=5) 83-91 87


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-Fetoprotein (AFP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Alpha-Fetoprotein (AFP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-105% 78-90% 91-105% 98-105%
EDTA plasma(n=5) 97-105% 80-93% 87-96% 90-103%
heparin plasma(n=5) 99-105% 89-98% 92-99% 90-97%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
BioFactors Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model PubMed: 20872761
Food and Nutrition Sciences Investigation of the Biochemical and Histological Changes Induced by Zearalenone Mycotoxin on Liver in Male Mice and the Protective Role of Crude Venom Extracted from Jellyfish Cassiopea Andromeda. Scirp: 5684
Diabetes Research and Clinical Practice Polyol profile as an early diagnostic and prognostic marker in natural product chemoprevention of hepatocellular carcinoma in diabetic rats ScienceDirect: S0168822711000520
Life Sciences Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver ScienceDirect: S0024320511002700
Annals of Hepatology Cytokines as important playmakers of experimental hepatocarcinogenesis confounded by diabetes. Hepatology: source
Biomedicine & Aging Pathology Chemopreventive and therapeutic efficacy of Salsola inermis extract against N-nitrosodiethylamine-initiated and phenobarbital-promoted hepatocellular carcinogenesis in Wistar rats ScienceDirect: S2210522013000348
European journal of pharmacology Suramin inhibits hepatic tissue damage in hepatocellular carcinoma through deactivation of heparanase enzyme Pubmed: 24530413
Asian Pac J Cancer Prev. Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. Pubmed:25684529
Journal of Analytical Atomic Spectrometry Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry Rsc:Source
PLOS ONE Development of a Highly Sensitive Glycan Microarray for Quantifying AFP-L3 for Early Prediction of Hepatitis B Virus–Related Hepatocellular Carcinoma Pubmed:24927126
BMC Med Imaging Evaluation of 11C-acetate and 18F-FDG PET/CT in mouse multidrug resistance gene-2 deficient mouse model of hepatocellular carcinoma PubMed: 25981587
Tumour Biol Elevated serum soluble CD14 levels in chronic HBV infection are significantly associated with HBV-related hepatocellular carcinoma PubMed: 26643893
Acta Veterinaria Brno The concentrations of selected blood serum proteins in calves during the first three months of life 85:33
Hepatobiliary Pancreat Dis Int Predictive value of tumor markers in patients with recurrent hepatocellular carcinoma in different vascular invasion pattern abstract:abstract4525.shtml
Journal of Applied Pharmaceutical Science Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats 2071_pdf.pdf
Frontiers in Physiology  Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats v04n07a07a
International Journal of Clinical and Experimental Medicine Effect of Mesenchymal Stem Cells on Transforming Growth Factor Beta Level in Hepatocellular Carcinoma Induced Rat Model 1345-1349
Tumor Biology Antiangiogenic activity of vitexicarpine in experimentally induced hepatocellular carcinoma: Impact on vascular endothelial growth factor pathway pubmed:28651490
Advances in Clinical and Experimental Medicine Mesenchymal stem cell therapy of hepatocellular carcinoma in rats: Detection of cell homing and tumor mass by magnetic resonance imaging using iron oxide nanoparticles ISSN 2451-2680
Tumor biology Evaluation of the antitumor activity of platinum nanoparticles in the treatment of hepatocellular carcinoma induced in rats pubmed:28720064
EBioMedicine Lnc-PCDH9-13: 1 Is a Hypersensitive and Specific Biomarker for Early Hepatocellular Carcinoma Pubmed:30045829
Sensors and Actuators B: Chemical One-pot synthesis of a highly selective carboxyl-functionalized superparamagnetic probes for detection of alpha-fetoprotein 10.1016:j.snb.2018.03.038
Biomedicine & Pharmacotherapy Cytotoxic and partial hepatoprotective activity of sodium ascorbate against hepatocellular carcinoma through inhibition of sulfatase-2 and Pubmed:29669302
Journal of Investigative Surgery Dynamic Volume Assessment of Hepatocellular Carcinoma in Rat Livers Using a Clinical 3T MRI and Novel Segmentation Pubmed:28107094
Asian Pacific Journal of Cancer Prevention Impact of Mesenchymal Stem Cells and Vitamin D on Transforming Growth Factor Beta Signaling Pathway in Hepatocellular Carcinoma in Rats Pubmed:29693337
Antioxid Redox Signal The Association of Peroxiredoxin 4 with the Initiation and Progression of Hepatocellular Carcinoma Pubmed:29687726
Cancer Biology Chemoprotective Efficacy of Curcumin and Flax Seed Oil in 1, 2-Dimethylhydrazine Induced Rat Colon Cancer
Cancer Biology Ameliorative effects of honey and venom of honey bee on induced colon cancer in male albino rats by1, 2 dimethylhydrazine
Biomedicine & Pharmacotherapy The therapeutic effects of nicotinamide in hepatocellular carcinoma through blocking IGF-1 and effecting the balance between Nrf2 and PKB Pubmed: 30784932
Asian Journal of Pharmacy and Pharmacology Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma
American Journal of Transplantation Circulating exosomal miR‐92b: Its role for cancer immunoediting and clinical value for prediction of posttransplant hepatocellular carcinoma recurrence Pubmed: 31162867
Environ Toxicol Pharmacol. Targetingp53/TRAIL/caspase-8 signaling by adiponectin reverses thioacetamide-induced hepatocellular carcinoma in rats Pubmed: 31421311
Hepatology Research Glutamine synthetase promotes tumor invasion in hepatocellular carcinoma through mediating epithelial–mesenchymal transition Pubmed: 31652385
REDOX REPORT Selective cytotoxic activity and protective effects of sodium ascorbate against hepatocellular carcinoma through its effect on oxidative stress and apoptosis in vivo and … Pubmed: 32172678
Scientific Reports The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study Pubmed: 32198440
Nutr Cancer Fucoidan Ameliorates Hepatocellular Carcinoma Induced in Rats: Effect on miR143 and Inflammation Pubmed: 32718197
Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles
Microchemical Journal Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS
Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats
Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo
DEVELOPMENT Blocking estrogen-induced AMH expression is crucial for normal follicle formation 33658225
Cytokine QNZ alleviated hepatocellular carcinoma by targeting inflammatory pathways in a rat model 34564023
Journal of Gastrointestinal Cancer In Vivo Study of a Newly Synthesized Chromen-4-One Derivative as an Antitumor Agent Against HCC 34698995
Catalog No. Related products for research use of Equus caballus; Equine (Horse) Organism species Applications (RESEARCH USE ONLY!)
RPA153Eq01 Recombinant Alpha-Fetoprotein (AFP) Positive Control; Immunogen; SDS-PAGE; WB.
PAA153Eq01 Polyclonal Antibody to Alpha-Fetoprotein (AFP) WB; IHC; ICC; IP.
MAA153Eq21 Monoclonal Antibody to Alpha-Fetoprotein (AFP) WB; IHC; ICC; IP.
SEA153Eq ELISA Kit for Alpha-Fetoprotein (AFP) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA153Eq Multiplex Assay Kit for Alpha-Fetoprotein (AFP) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.