ELISA Kit for Interferon Beta (IFNb)

IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast


This assay has high sensitivity and excellent specificity for detection of Interferon Beta (IFNb).
No significant cross-reactivity or interference between Interferon Beta (IFNb) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Interferon Beta (IFNb) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Beta (IFNb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-101 97
EDTA plasma(n=5) 88-104 99
heparin plasma(n=5) 79-104 86


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Beta (IFNb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Beta (IFNb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-90% 94-101% 82-99% 93-102%
EDTA plasma(n=5) 78-89% 80-91% 87-97% 85-105%
heparin plasma(n=5) 86-101% 94-102% 98-105% 80-90%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
20 Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway PubMed: 21355876
Journal of General Virology Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. Sgm: 9182080
Biochemical and Biophysical Research Communications Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage ScienceDirect: S0006291X09019822
PLoS One Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys PubMed: PMC3757038
PLoS Neglected Tropical Diseases Innate Immune Response to Rift Valley Fever Virus in Goats PubMed: PMC3335883
BioMed Research International Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages Pubmed: 24288685
Vet Microbiol. An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. Pubmed: 23850441
Food and Chemical Toxicology Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice Pubmed: 23535186
Veterinary immunology and immunopathology Experimental model of equine alveolar macrophage stimulation with TLR ligands Pubmed: 23815824
American journal of respiratory cell and molecular biology Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages Pubmed: 24502337
FEBS Lett. Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity Pubmed:24530528
Veterinary Research Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain Pubmed:25106750
PLOS Neglected Tropical Diseases MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production Pubmed:25122182
Veterinary Research The innate immune response of equine bronchial epithelial cells is altered by training Pubmed:25595212
molecular and celluar biology SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity PubMed: 25939384
Arch Virol Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus PubMed: 25797196
J Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells PubMed: 26258404
Virus PKR activation enhances replication of classical swine fever virus in PK-15 cells PubMed: 25899421
PLoS One siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro Pubmed:26886455
Scientific Reports Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 Pubmed:27090571
Front Microbiol Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo Pubmed:27252695
Canadian Journal of Physiology and Pharmacology Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. pubmed:28134560
International Journal of Molecular Medicine Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function pubmed:27176664
International Journal of Clinical and Experimental Pathology Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells files:ijcep0036479.pdf
Poultry Science ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages pubmed:27486255
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
Biochemical and Biophysical Research Communications MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B pubmed:28342861
Virology The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection pubmed:28988058
EMBO Journal Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis Pubmed:30065070
Toxicology and applied pharmacology Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice Pubmed:30017639
Archives of Virology MAVS induces a host cell defense to inhibit CSFV infection Pubmed:29556776
Journal of virology Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation Pubmed: 30518647
Veterinary Immunology and Immunopathology Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… Doi: 10.1016/j.vetimm.2019.01.003
Science Signaling HIPK2 is necessary for type I interferon–mediated antiviral immunity Doi: 10.1126/scisignal.aau4604
INTERNATIONAL MEDICAL RESEARCH Evaluation of serum interferons in patients with age-related macular degeneration Pubmed: 31007694
Veterinary Immunology and Immunopathology Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
International journal of biological macromolecules Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction Pubmed: 31284005
Journal of virology Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation Pubmed: 31243133
The Journal of Latvian Academy of Sciences Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia
Cancer Medicine The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 Pubmed: 31218844
FASEB Journal Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
PLOS Pathogens Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion Pubmed: 31539404
Autophagy Induction of autophagy and suppression of type I IFN secretion by CSFV Pubmed: 32160078
MOLECULAR IMMUNOLOGY BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation Pubmed: 33053462
Autophagy LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection Pubmed: 32924761
Arch Virol . Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway Pubmed: 32532134
Virulence Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
bioRxiv Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance
Res Vet Sci Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells 34416463
Eur J Immunol Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity Pubmed:35419822
ResearchSquare Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
EPA222Mu61 Eukaryotic Interferon Beta (IFNb) Positive Control; Immunogen; SDS-PAGE; WB.
APA222Mu61 Active Interferon Beta (IFNb) Cell culture; Activity Assays.
RPA222Mu02 Recombinant Interferon Beta (IFNb) Positive Control; Immunogen; SDS-PAGE; WB.
APA222Mu01 Active Interferon Beta (IFNb) Cell culture; Activity Assays.
RPA222Mu01 Recombinant Interferon Beta (IFNb) Positive Control; Immunogen; SDS-PAGE; WB.
PAA222Mu02 Polyclonal Antibody to Interferon Beta (IFNb) WB; IHC; ICC; IP.
PAA222Mu01 Polyclonal Antibody to Interferon Beta (IFNb) WB; IHC; ICC; IP.
SEA222Mu ELISA Kit for Interferon Beta (IFNb) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA222Mu Mini Samples ELISA Kit for Interferon Beta (IFNb) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA222Mu Multiplex Assay Kit for Interferon Beta (IFNb) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.