ELISA Kit for Urocortin (UCN)

UI; UROC; Urocortin 1; UCN1


This assay has high sensitivity and excellent specificity for detection of Urocortin (UCN).
No significant cross-reactivity or interference between Urocortin (UCN) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Urocortin (UCN) and the recovery rates were calculated by comparing the measured value to the expected amount of Urocortin (UCN) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 83-90 86
EDTA plasma(n=5) 95-103 98
heparin plasma(n=5) 91-98 94


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Urocortin (UCN) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Urocortin (UCN) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Urocortin (UCN) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 92-102% 80-101% 81-102% 98-105%
EDTA plasma(n=5) 91-105% 96-105% 96-104% 81-103%
heparin plasma(n=5) 78-92% 79-104% 93-105% 80-97%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Human Reproduction P-205 Basement membrane integrity is altered in the late secretary phase in women with endometriosis: implications for the pathogenesis of endometriosis OxfordJournals: source
PLOS ONE Vasoprotective effects of urocortin 1 against atherosclerosis in vitro and in vivo Pubmed:25462164
Taiwan J Obstet Gynecol Novel markers in the diagnostics of endometriomas: Urocortin, ghrelin, and leptin or leukocytes, fibrinogen, and CA-125? PubMed: 25951715
Biomedical Journal of Scientific & Technical Research Anti-Inflammatory and Pro-Inflammatory Neuropeptides in Dilative Cardiomyopathy are there Relations to Psychophysical Stress?
Neurobiol Stress Erasing m6A-dependent transcription signature of stress-sensitive genes triggers antidepressant actions 34527794
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA231Ra01 Recombinant Urocortin (UCN) Positive Control; Immunogen; SDS-PAGE; WB.
CPA231Ra21 OVA Conjugated Urocortin (UCN) Immunogen; SDS-PAGE; WB.
PAA231Ra08 Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
PAA231Ra01 Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
LAA231Ra71 Biotin-Linked Polyclonal Antibody to Urocortin (UCN) WB; IHC; ICC.
MAA231Ra21 Monoclonal Antibody to Urocortin (UCN) WB; IHC; ICC; IP.
SEA231Ra ELISA Kit for Urocortin (UCN) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA231Ra Multiplex Assay Kit for Urocortin (UCN) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.