ELISA Kit for Trefoil Factor 2 (TFF2)

SML1; SP; Spasmolytic Protein 1; Spasmolysin; Spasmolytic polypeptide


This assay has high sensitivity and excellent specificity for detection of Trefoil Factor 2 (TFF2).
No significant cross-reactivity or interference between Trefoil Factor 2 (TFF2) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Trefoil Factor 2 (TFF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Trefoil Factor 2 (TFF2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-99 95
EDTA plasma(n=5) 84-93 89
heparin plasma(n=5) 87-105 98


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Trefoil Factor 2 (TFF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Trefoil Factor 2 (TFF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Trefoil Factor 2 (TFF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-97% 80-89% 84-105% 92-101%
EDTA plasma(n=5) 83-104% 85-92% 80-103% 95-104%
heparin plasma(n=5) 78-95% 81-97% 95-103% 90-97%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
BMC Gastroenterology Serum trefoil factor 3 is a promising non-invasive biomarker for gastric cancer screening: A monocentric cohort study in China Biomedcentral: Source
PLoS One Obstructive sleep apnea and rhonchopathy are associated with downregulation of trefoil factor family peptide 3 (TFF3)-Implications of changes in oral mucus composition. pubmed:29028798
Scientific Reports Exocrine tissue-driven TFF2 prevents apoptotic cell death of endocrine lineage during pancreas organogenesis Pubmed: 30733468
Mucosal Immunology ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism Pubmed: 31278375
International Journal of Molecular Sciences Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1–3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid …
Int. J. Mol. Sci. Untersuchungen der Trefoil factor family Peptide 1-3 in humaner Synovialfl¨¹ssigkeit und Synovialmembran und deren Bedeutung bei Arthrose und?¡­
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA748Hu01 Recombinant Trefoil Factor 2 (TFF2) Positive Control; Immunogen; SDS-PAGE; WB.
APA748Hu01 Active Trefoil Factor 2 (TFF2) Cell culture; Activity Assays.
PAA748Hu01 Polyclonal Antibody to Trefoil Factor 2 (TFF2) WB; IHC; ICC; IP.
LAA748Hu71 Biotin-Linked Polyclonal Antibody to Trefoil Factor 2 (TFF2) WB; IHC; ICC.
MAA748Hu22 Monoclonal Antibody to Trefoil Factor 2 (TFF2) WB; IHC; ICC; IP.
SEA748Hu ELISA Kit for Trefoil Factor 2 (TFF2) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA748Hu Multiplex Assay Kit for Trefoil Factor 2 (TFF2) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.