ELISA Kit for Cluster Of Differentiation 26 (CD26)

CD26; ADA; DPPIV; DPP-IV; DPP4; ADABP; ADCP2; TP103; Adenosine Deaminase Complexing Protein 2; T-cell activation antigen CD26; Dipeptidyl Peptidase IV


This assay has high sensitivity and excellent specificity for detection of Cluster Of Differentiation 26 (CD26).
No significant cross-reactivity or interference between Cluster Of Differentiation 26 (CD26) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Cluster Of Differentiation 26 (CD26) and the recovery rates were calculated by comparing the measured value to the expected amount of Cluster Of Differentiation 26 (CD26) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 89-101 94
EDTA plasma(n=5) 82-97 93
heparin plasma(n=5) 97-105 101


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cluster Of Differentiation 26 (CD26) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Cluster Of Differentiation 26 (CD26) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-99% 88-97% 78-103% 95-105%
EDTA plasma(n=5) 97-105% 98-105% 97-105% 94-101%
heparin plasma(n=5) 95-105% 81-99% 83-104% 79-92%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
hormone and metabolic research Linagliptin Blocks Renal Damage in Type 1 Diabetic Rats by Suppressing Advanced Glycation End Products-Receptor Axis Pubmed: 24710699
Curr Med Chem. MK-0626, a dipeptidyl peptidase-4 inhibitor, improves neovascularization by increasing both the number of circulating endothelial progenitor cells and endothelial nitric oxide synthetase expression. Pubmed:24059225
Diabetes Ghrelin inhibition restores glucose homeostasis in hepatocyte nuclear factor-1alpha (MODY3) deficient mice PubMed: 25979074
The American Journal of the Medical Sciences Expression and Clinical Significance of Serum Dipeptidyl Peptidase IV Chronic Obstructive Pulmonary Disease Pubmed:26992252
Gene Expression of recombinant human α-lactalbumin in milk of transgenic cloned pigs is sufficient to enhance intestinal growth and weight gain of suckling piglets Pubmed:26899869
Applied In Vitro Toxicology Hepatic Spheroids for Long-Term Toxicity Studies aivt.2016.0016
BMJ Open Diab Res Care High circulating plasma dipeptidyl peptidase- 4 levels in non-obese Asian Indians with type 2 diabetes correlate with fasting insulin and LDL-C levels, triceps skinfolds, total intra-abdominal adipose tissue volume and presence of diabetes: a case–control study 10.1136:bmjdrc-2017-000393
Diabetology & Metabolic Syndrome Dipeptidyl peptidase-4 levels are increased and partially related to body fat distribution in patients with familial partial lipodystrophy type 2 pubmed:28450900
Journal of Cellular Biochemistry The effect of CD26‐deficiency on dipeptidyl peptidase 8 and 9 expression profiles in a mouse model of Crohn's disease Pubmed:29693275
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity Pubmed:29953010
Sci Rep The novel adamantane derivatives as potential mediators of inflammation and neural plasticity in diabetes mice with cognitive impairment Pubmed:35468904
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPA884Ra01 Recombinant Cluster Of Differentiation 26 (CD26) Positive Control; Immunogen; SDS-PAGE; WB.
PAA884Ra01 Polyclonal Antibody to Cluster Of Differentiation 26 (CD26) WB; IHC; ICC; IP.
LAA884Ra71 Biotin-Linked Polyclonal Antibody to Cluster Of Differentiation 26 (CD26) WB; IHC; ICC.
MAA884Ra21 Monoclonal Antibody to Cluster Of Differentiation 26 (CD26) WB; IHC; ICC; IP.
SEA884Ra ELISA Kit for Cluster Of Differentiation 26 (CD26) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA884Ra Multiplex Assay Kit for Cluster Of Differentiation 26 (CD26) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.