ELISA Kit for Sulfotransferase 1A1 (SULT1A1)

PST; HAST1/HAST2; ST1A3; STP1; TSPST1; Phenol Sulfotransferase; Aryl sulfotransferase 1; Phenol sulfotransferase 1; Thermostable phenol sulfotransferase


This assay has high sensitivity and excellent specificity for detection of Sulfotransferase 1A1 (SULT1A1).
No significant cross-reactivity or interference between Sulfotransferase 1A1 (SULT1A1) and analogues was observed.


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfotransferase 1A1 (SULT1A1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Reproductive Toxicology Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. PubMed: 22750137
The Journal of Nutrition Apiaceous Vegetable Consumption Decreases PhIP-Induced DNA Adducts and Increases Methylated PhIP Metabolites in the Urine Metabolome in Rats1,2,4 PubMed: 25733458
Molecular Nutrition Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats Pubmed:27133590
Molecular Nutrition & Food Research Phenethyl isothiocyanate and indole-3-carbinol from cruciferous vegetables, but not furanocoumarins from apiaceous vegetables, reduced PhIP-induced DNA adducts in Wistar rats. pubmed:27133590
Redox Biology STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. pubmed:29078168
Available at SSRN 3234834 Human Plasma Protein (s) Based Conceptual Diagnostic Tool for Altitude-Acclimation Assessment
Life sciences Reverse translating SULT1A1, a potential biomarker in roentgenographically tested rat model of rapid HAPE induction Pubmed: 31100325
Functional & Integrative Genomics Plasma protein (s)–based conceptual diagnostic tool for assessing high-altitude acclimation in humans Pubmed: 31444657
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