ELISA Kit for Troponin T Type 2, Cardiac (TNNT2)

CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T


This assay has high sensitivity and excellent specificity for detection of Troponin T Type 2, Cardiac (TNNT2).
No significant cross-reactivity or interference between Troponin T Type 2, Cardiac (TNNT2) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Troponin T Type 2, Cardiac (TNNT2) and the recovery rates were calculated by comparing the measured value to the expected amount of Troponin T Type 2, Cardiac (TNNT2) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-97 94
EDTA plasma(n=5) 80-98 87
heparin plasma(n=5) 96-103 99


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Troponin T Type 2, Cardiac (TNNT2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Troponin T Type 2, Cardiac (TNNT2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-103% 91-101% 85-103% 91-104%
EDTA plasma(n=5) 85-96% 79-92% 78-104% 91-99%
heparin plasma(n=5) 91-98% 95-104% 94-104% 85-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of Sulphonated Formononetin on Acute Myocardial Infarction in Rats PubMed: 21232020
Basic & Clinical Pharmacology & Toxicology Cardioprotective Effect of SMND-309, A Novel Derivate of Salvianolic Acid B on Acute Myocardial Infarction in Rats Wiley: source
European Journal of Pharmacology Protective roles of Asperosaponin VI, a triterpene saponin isolated from Dipsacus asper Wall on acute myocardial infarction in rats ScienceDirect: S0014299909009984
Journal of translational medicine The altered expression profile of microRNAs in cardiopulmonary bypass canine models and the effects of mir-499 on myocardial ischemic reperfusion injury PubMed: PMC3694448
Oral Diseases Salivary high‐sensitivity cardiac troponin T levels in patients with acute myocardial infarction Pubmed: 22834943
An International Journal of Pharmaceutical Sciences In vivo and in vitro cardioprotective effects of Panax quinquefolium 20 (S)-protopanaxadiol saponins (PQDS), isolated from Panax quinquefolium Pubmed: 23700796
Journal of Animal and Feed Sciences, The effects of caloric restriction and age on thyroid hormone signalling in the heart of rats Pl:Source
Liver Int. Cardiomyopathy reverses with recovery of liver injury, cholestasis and cholanemia in mouse model of biliary fibrosis Pubmed:24330504
Cardiovasc Toxicol. Cardioprotective Effect of Rhizomes of Acorus gramineus Against Isoproterenol-Induced Cardiac Damage in Pigs Pubmed:24420420
British Journal of Pharmacology Glutathione administration reduces mitochondrial damage and shifts cell death from necrosis to apoptosis in ageing diabetic mice hearts during exercise Pubmed:25039894
Chemico-Biological Interactions Crocin protects against doxorubicin-induced myocardial toxicity in rats through down-reCavia (Guinea pig )lation of inflammatory and apoptic pathways Pubmed:26807765
The Journal of Pharmacology and Experimental Therapeuties The Cardenolide Glycoside Acovenoside A Affords Protective Activity in Doxorubicin-Induced Cardiotoxicity in Mice Pubmed:27247000
The Journal of Maternal-Fetal & Neonatal Medicine Troponins, heat shock proteins and glycogen phosphorylase BB in umbilical cord blood of complicated pregnancies. pubmed:27937003
PLoS One. Outer Balloon Ligation Increases Success Rate of Ischemia-Reperfusion Injury Model in Mice. pubmed:27907155
Biomedicine & Pharmacotherapy Rosuvastatin improves myocardial and neurological outcomes after asphyxial cardiac arrest and cardiopulmonary resuscitation in rats pubmed:28076830
PLoS One Imatinib attenuates cardiac fibrosis by inhibiting platelet-derived growth factor receptors activation in isoproterenol induced model pubmed:28570599
EXPERIMENTAL AND THERAPEUTIC MEDICINE Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus pubmed:29042932
PLoS One Increases in plasma corin levels following experimental myocardial infarction reflect the severity of ischemic injury Pubmed: 30192780
PLoS One Tissue suction-mediated gene transfer to the beating heart in mice Pubmed: 32027678
Journal of Human Kinetics Hormonal Response to Incremental and Continuous Exercise in Cyclists with Left Ventricle Hypertrophy Pubmed: 32148580
BIOMEDICINE & PHARMACOTHERAPY Chlorogenic acid prevents acute myocardial infarction in rats by reducing inflammatory damage and oxidative stress Pubmed: 33022535
Toxics Norepinephrine Leads to More Cardiopulmonary Toxicities than Epinephrine by Catecholamine Overdose in Rats Pubmed: 32947820
Elife Iron derived from autophagy-mediated ferritin degradation induces cardiomyocyte death and heart failure in mice 33526170
Cell Metabolism Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species 34536344
Anal Bioanal Chem Development of a novel ssDNA aptamer targeting cardiac troponin I and its clinical applications 34673993
Experimental and Therapeutic Medicine Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest Pubmed:35495584
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