ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A)

PLA2; MOM1; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2(platelets,synovial fluid); Non-pancreatic secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A


This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group IIA (PLA2G2A).
No significant cross-reactivity or interference between Phospholipase A2, Group IIA (PLA2G2A) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Phospholipase A2, Group IIA (PLA2G2A) and the recovery rates were calculated by comparing the measured value to the expected amount of Phospholipase A2, Group IIA (PLA2G2A) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 91-99 94


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Group IIA (PLA2G2A) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Group IIA (PLA2G2A) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Phospholipase A2, Group IIA (PLA2G2A) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-103% 81-101% 86-102% 93-101%
EDTA plasma(n=5) 84-94% 92-101% 99-105% 92-101%
heparin plasma(n=5) 86-94% 98-105% 97-105% 87-102%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Journal of Nephropathology Anti-phospholipase A2 receptor antibody in idiopathic membranous nephropathy: A report from Iranian population NCBI: PMC3891130
Journal of Nutritional Science Yogurt drink fortified with menaquinone-7 improves vitamin K status in a healthy population PubMed: 26495126
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
RPD827Ra01 Recombinant Phospholipase A2, Group IIA (PLA2G2A) Positive Control; Immunogen; SDS-PAGE; WB.
PAD827Ra01 Polyclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A) WB; IHC; ICC; IP.
MAD827Ra21 Monoclonal Antibody to Phospholipase A2, Group IIA (PLA2G2A) WB; IHC; ICC; IP.
SED827Ra ELISA Kit for Phospholipase A2, Group IIA (PLA2G2A) Enzyme-linked immunosorbent assay for Antigen Detection.
LMD827Ra Multiplex Assay Kit for Phospholipase A2, Group IIA (PLA2G2A) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.