ELISA Kit for Semaphorin 3E (SEMA3E)

SEMAH; M-SemaK; M-sema H; coll-5; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3E


This assay has high sensitivity and excellent specificity for detection of Semaphorin 3E (SEMA3E).
No significant cross-reactivity or interference between Semaphorin 3E (SEMA3E) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Semaphorin 3E (SEMA3E) and the recovery rates were calculated by comparing the measured value to the expected amount of Semaphorin 3E (SEMA3E) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-94 84
EDTA plasma(n=5) 79-90 85
heparin plasma(n=5) 87-99 93


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Semaphorin 3E (SEMA3E) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Semaphorin 3E (SEMA3E) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 83-91% 78-97% 86-98%
EDTA plasma(n=5) 87-101% 83-104% 93-103% 86-104%
heparin plasma(n=5) 83-92% 85-96% 96-103% 85-99%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Ophthalmology Aqueous Levels of Angiopoietin-like 4 and Semaphorin 3E Correlate with Nonperfusion Area and Macular Volume in Diabetic Retinopathy PubMed: 25687026
annals of clinical and laboratory science Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome. pubmed:28249916
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE Spatial association of SEMA3C with nerve endings/terminal Schwann cells in hair follicle isthmus region Pubmed: 32954569
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY The effect of nutrition and exercise training on irisin and semaphorin-3E levels in obese patients Pubmed: 32569480
EMBO JOURNAL RhoJ integrates attractive and repulsive cues in directional migration of endothelial cells Pubmed: 32347571
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPL920Hu01 Recombinant Semaphorin 3E (SEMA3E) Positive Control; Immunogen; SDS-PAGE; WB.
PAL920Hu01 Polyclonal Antibody to Semaphorin 3E (SEMA3E) WB; IHC; ICC; IP.
MAL920Hu21 Monoclonal Antibody to Semaphorin 3E (SEMA3E) WB; IHC; ICC; IP.
SEL920Hu ELISA Kit for Semaphorin 3E (SEMA3E) Enzyme-linked immunosorbent assay for Antigen Detection.
LML920Hu Multiplex Assay Kit for Semaphorin 3E (SEMA3E) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.