Wide-range ELISA Kit for Anti-Mullerian Hormone (AMH)

MIF; MIH; MIS; Müllerian Inhibiting Factor; Müllerian Inhibiting Hormone; Müllerian Inhibiting Substance


This assay has high sensitivity and excellent specificity for detection of Wide-range Anti-Mullerian Hormone (AMH).
No significant cross-reactivity or interference between Wide-range Anti-Mullerian Hormone (AMH) and analogues was observed.


Matrices listed below were spiked with certain level of recombinant Wide-range Anti-Mullerian Hormone (AMH) and the recovery rates were calculated by comparing the measured value to the expected amount of Wide-range Anti-Mullerian Hormone (AMH) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 93-101 97
EDTA plasma(n=5) 78-97 83
heparin plasma(n=5) 80-97 79


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Anti-Mullerian Hormone (AMH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Anti-Mullerian Hormone (AMH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Wide-range Anti-Mullerian Hormone (AMH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 96-104% 90-104% 79-90%
EDTA plasma(n=5) 90-102% 85-92% 96-104% 89-97%
heparin plasma(n=5) 88-104% 94-101% 87-94% 95-103%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.



Magazine Citations
Journal of Zhejiang University SCIENCE B Influence of gonadotropin-releasing hormone agonist on the effect of chemotherapy upon ovarian cancer and the prevention of chemotherapy-induced ovarian damage: an experimental study with nu/nu athymic mice. PubMed: 23125082
In Vivo.(Athens,Greece) Association Between AMH, Oocyte Number and Availability of Embryos for Cryopreservation in IVF Pubmed: 24292596
Gynecological Endocrinology The effects of isotretinoin on the ovarian reserve of females with acne Informahealthcare: Source
Fertility and Sterility Increased expression of anti mullerian hormone and its receptor in endometriosis Ingentaconnect: Source
Reproductive BioMedicine Online Decreased ovarian reserve in female Sprague–Dawley rats induced by isotretinoin (retinoic acid) exposure Rbmojournal: Source
Reproductive Biology and Endocrinology The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study. Pubmed: 24341292
Biomed Res Int. Oxytocin Improves Follicular Reserve in a Cisplatin-Induced Gonadotoxicity Model in Rats Pubmed:Pmc4053297
Fertil Steril. Increased expression of antimüllerian hormone and its receptor in endometriosis Pubmed:24613539
The Journal of Maternal-Fetal &amp; Neonatal Medicine Maternal serum anti-Müllerian hormone at 11-13 weeks&#039; gestation in the prediction of preeclampsia Pubmed:24953352
J Gynecol Oncol. Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model Pubmed:25142624
Reproductive BioMedicine Online The effect of childhood cancer therapy on ovarian reserve and pubertal development Pubmed:25498597
Reproductive Sciences Effects of Resveratrol on Ovarian Morphology, Plasma Anti-Mullerian Hormone, IGF-1 Levels, and Oxidative Stress Parameters in a Rat Model of Polycystic Ovary Syndrome Pubmed:25667201
Yonsei Medical Journal Anti-Proliferative and Apoptotic Activities of Müllerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines PubMed: 26632380
oncology research Mechanistic Study on Triptorelin Action in Protecting From 5-FU-Induced Ovarian Damage in Rats PubMed: 26629940
Reprod Biomed Online Protective effect of resveratrol against oxidative damage to ovarian reserve in female Sprague–Dawley rats PubMed: 26206282
PLoS One Growth Hormone Ameliorates the Radiotherapy-Induced Ovarian Follicular Loss in Rats: Impact on Oxidative Stress, Apoptosis and IGF-1/IGF-1R Axis PubMed: 26465611
Toxicol Lett Long-term effects of methamphetamine exposure in adolescent mice on the future ovarian reserve in adulthood PubMed: 26657179
Journal of Assisted Reproduction and Genetics Can Coenzyme Q10 supplementation protect the ovarian reserve against oxidative damage? Pubmed:27255570
Anticancer Res. Prognostic Value of Anti-Müllerian Hormone and Inhibin B in Patients with Premenopausal Hormone Receptor-positive Breast Cancer Pubmed:26976997
Chemosphere. Gestational and lactational exposure to bisphenol AF in maternal rats increases testosterone levels in 23-day-old male offspring. pubmed:27567155
Theriogenology Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males. pubmed:28043339
environmental pollution Environmental exposure to polycyclic aromatic hydrocarbons (PAHs): The correlation with and impact on reproductive hormones in umbilical cord serum. pubmed:27838061
Journal of Assisted Reproduction and Genetics Intrafollicular inflammatory cytokines but not steroid hormone concentrations are increased in naturally matured follicles of women with proven endometriosis. pubmed:28074436
Journal Pakistan Medical Association Serum anti-mullerian hormone: Correlation with the ovarian follicular dynamics in healthy mice pubmed:27654725
Advance Medical Associates Are anti-Müllerian hormone and its receptor polymorphism associated with the hormonal condition of undescended testes? pubmed:27162065
Yonsei Med J Volume Anti-Proliferative and Apoptotic Activities of M? llerian Inhibiting Substance Combined with Calcitriol in Ovarian Cancer Cell Lines. pubmed:26632380
Journal of Endocrinological Investigation The use of serum anti-Mullerian hormone (AMH) levels and antral follicle count (AFC) to predictthe number of oocytes collected and availability of embryos for cryopreservation in IVF. pubmed:27465668
Journal of the Pakistan Medical Association Predictive accuracy of anti mullerian hormone as indicator of ovarian follicle loss in cyclophosphamide treated mice pubmed:28955058
International Journal of Reproduction, Contraception, Obstetrics and Gynecology Can Co-enzyme Q10 improve the chances of conception after the age of 35? 10.18203/2320-1770.ijrcog20172902
Animal Production Science Can serum levels of anti-Müllerian hormone and oestradiol in juvenile gilts be used to predict future reproductive performance? 10.1071/ANv57n12Ab065
Reproduction. Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring pubmed:28687593
Theriogenology Relationship among circulating anti-Müllerian hormone, insulin like growth factor 1, cadmium and superovulatory response in dairy cows. pubmed:28708536
Journal of ovarian research Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo. pubmed:28814333
Annals of Rheumatic Diseases  FRI0390 Impaired ovarian reserve in patients affected by systemic lupus erythematosus: a case-control study Suppl_2:728.1.abstract
Free Radical Biology & Medicine Peroxiredoxin 2 deficiency accelerates age-related ovarian failure through the reactive oxygen species-mediated JNK pathway in mice Pubmed:29778464
Environmental Pollution Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population Pubmed:29525625
Biology of Reproduction Maternal nutrient restriction impairs young adult offspring ovarian signaling resulting in reproductive dysfunction and follicle loss Pubmed:29351580
Endocrine Connections Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recovery Pubmed:30012648
Reproductive BioMedicine Online Dienogest suppresses the activation of primordial follicles and preserves the primordial follicle stockpile for fertility in mice Pubmed:29609766
Journal of Ovarian Research Comparison of the influence of three fibroid treatment options: supracervical hysterectomy, ulipristal acetate and uterine artery embolization on ovarian … Pubmed:29859107
Animal Reproduction Science Anti-Müllerian hormone and Oestradiol as markers of future reproductive success in juvenile gilts Pubmed:29859701
Stem Cell Research & Therapy Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the … Pubmed: 30606243
Biochemical and Biophysical Research Communications Involvement of obesity-associated upregulation of chemerin/chemokine-like receptor 1 in oxidative stress and apoptosis in ovaries and granulosa cells Pubmed: 30722991
Lupus Levels of anti-Müllerian hormone in premenopausal women with the antiphospholipid syndrome and its association with the risk of clinical complications Pubmed: 30717622
In Vitro Cellular & Developmental Biology - Animal Transplantation of umbilical cord–derived mesenchymal stem cells on a collagen scaffold improves ovarian function in a premature ovarian failure model of mice Pubmed: 30820812
Biomedicine & Pharmacotherapy Protective properties of glycogen synthase kinase-3 inhibition against doxorubicin-induced oxidative damage to mouse ovarian reserve
Reproductive Biology and Endocrinology Menstrual blood derived mesenchymal stem cells combined with Bushen Tiaochong recipe improved chemotherapy-induced premature ovarian failure in … Pubmed: 31311554
Oxidative Medicine and Cellular Longevity Carvacrol and Thymol Modulate the Cross-Talk between TNF-α and IGF-1 Signaling in Radiotherapy-Induced Ovarian Failure
Acta Obstet Gynecol Scand The impact of uterine artery embolization on ovarian reserve: a systematic review and meta‐analysis Pubmed: 31370100
Reproductive Sciences Uterine Cells Improved Ovarian Function in a Murine Model of Ovarian Insufficiency Pubmed: 31530098
Animals Serum Concentrations of AMH and E2 and Ovarian and Uterine Traits in Gilts Pubmed: 31619004
Journal Of Pakistan Medical Association The effect of acupuncture on anti-mullerian hormone and assisted reproduction outcome in Polycystic Ovary Syndrome patients undergoing in vitro fertilization … Pubmed: 31603867
In Vitro Cellular & Developmental Biology Basal characterization and in vitro differentiation of putative stem cells derived from the adult mouse ovary Pubmed: 31900800
J Assist Reprod Genet The protective effect of platelet-rich plasma administrated on ovarian function in female rats with Cy-induced ovarian damage Pubmed: 32020412
Biology (Basel). Seminal Plasma Anti-Müllerian Hormone: A Potential AI-Boar Fertility Biomarker? Pubmed: 32290279
Eur J Breast Health The Effect of Systemic Chemotherapy on Ovarian Function: A Prospective Clinical Trial Pubmed: 32656517
Heliyon Optimized platelet rich plasma releasate (O-rPRP) repairs galactosemia-induced ovarian follicular loss in rats by activating mTOR signaling and inhibiting … Pubmed: 33005806
CYTOMETRY PART A After cyclophosphamide exposure, granulosa cells recover their anti©\M¨¹llerian hormone©\producing ability but not their numbers 33342073
BIOLOGY OF REPRODUCTION Igf3 is essential for ovary differentiation in zebrafish 33276384
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis 33422655
Stem Cells Int Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in … 34733331
Sci Total Environ Exposure to multiple pyrethroid insecticides affects ovarian follicular development via modifying microRNA expression Pubmed:35276145
Hum Reprod Update Anti-Müllerian hormone as a marker of ovarian reserve and premature ovarian insufficiency in children and women with cancer: a systematic review Pubmed:35199161
Horm Metab Res Anti-Mullerian Hormone Levels Increase After Bariatric Surgery in Obese Female Patients With and Without Polycystic Ovary Syndrome Pubmed:35276745
American Journal of Translational Research The effects of vasopressin injection technique on ovarian reserve in laparoscopic cystectomy of bilateral ovarian endometrioma: a retrospective cohort study Pubmed:35559399
Stem Cell Research & Therapy G-CSF-mobilized Peripheral Blood Mononuclear Cells Combined with Platelet-rich Plasma Restored the Ovarian Function of aged rats via angiogenesis and …
Research Square Drug-free in vitro activation combined 3D-bioprinted adipose-derived stem cells restore ovarian function of premature ovarian insufficiency
Fundamental Research Long-term amelioration of an early-onset familial atrial fibrillation model with AAV-mediated in vivo gene therapy
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