-
Certificate of Analysis--customized labeled peptide
[ PROUDUCT ] Lot.No.: P20151130397 Protein Name: CKGGRAKDC tagged to rhodamine Size: 1mg [ QC TESTING ] Relative abundance of peptide is list bellow: Spectrum peak of labled peptide is list bellow: Relative abundance of labled peptide is list bellow:
-
Principle for Immunohistochemistry (IHC) Assay
Immunohistochemistry (IHC) is also called as immunocytochemistry (ICC). It is an immunological method for qualitative and quantitative detection, as well as location of antigen in situ in cells of a tissue section, using specific and labeled antibody. Below is the principle:
-
Animal Experiment can be Customized, Really?
Animal experiment is an indispensable part in many scientific fields, such as Immunology, Pharmacy, Medicine, etc.. Many researchers might list lots of headaches when mentioned animal experiment, ethical inspection is time consuming, the quality of the animal is hard to guarantee, sample collection is very complicated.... Now, there is no need to worry about these problems. Uscn Experimental Animal Inc., which is the subordinate enterprises of Cloud-clone.Corp., can provide customized animal experiment service. What kinds of service could be expected? Currently, the services include: many kinds of open animal experiments, pharmacodynamics and pharmacokinetics experiments, medicine saf......
-
Quantitative Determination of Caspase-1 Expression in ATP and LPS Stimulated Monocytes by ELISA Analysis
Caspase family appears to be the key members involved in cellular apoptosis process and closely related with the morphological characteristics of apoptosis. It has been paid much attention by researchers during these years. Caspase-1 is the first discovered member, which was cloned and sequenced in 1992. After then, 14 Caspase members were cloned. Caspase-1, the key factor in the apoptosis mechanism, mainly involve in cytokine-mediated inflammatory response. Meanwhile, it plays a role in the death receptor-mediated cell apoptosis, taking part in the mature and transport of IL-1β, as well as related physiological and pathological process. During the stimulation of several factors s......
-
The Detailed Preparation Method of CD40L and CD40 in-vitro
CD40L (Cluster of differentiation 40 Ligand) and CD40 (Cluster of differentiation 40) are a pair of complementary co-stimulatory molecules, taking part in specific immune response system in body. They play important roles in antigen presentation and immune responses, the abnomality of either CD40L or CD40 will cause a series of downstream reactions CD40 is a member of the TNFR superfamily, which is a type I transmembrane protein mainly expressed in B cells. CD40, known as the APC (antigen presenting cells) co-stimulatory protein, is essential for the activation of APC. CD40 can combine with TRAF1, TRAF2, TRAF3, TRAF5, TRAF6 and TTRAP. CD40L was originally found on the surface of acti......
-
The Detailed Construction Method of recombinant protein
Recombinant protein is produced by transferring the target gene to a vector and expressed in host cells. It plays important roles in signal transduction, pharmaceutical development and scientific research. Below is an introduction on production process of recombinant proteins. 1.Gene amplification (1)Primers synthesis Primers are designed by analyzing the gene sequence as well as the protein structure. Trans-membrane area and complex secondary structures should be avoided. Meanwhile, it is preferred to choose N or C terminal sequence and the evaluation of the primers should be high. Search restriction sites and proper vector. (2)Gene cloning and identification of recombinan......