After recombinant protein Primer Pairs, Cloud-Clone Corp. released another molecular cloning tool --Taq DNA polymerase. Taq DNA polymerase was originally from Thermus Aquaticus (Taq) yT1 strains isolated from volcanic hot springs bacterium in Yellowstone National Forest Park in 1969. This enzyme gene contains 2496 bases, encoding 832 amino acids. It is a heat-stable DNA polymerases with molecular weight of approximately 94kDa. Its optimal operating temperature is 75-80°C. At conventional PCR extension phase (72°C), about 1000 nucleotides can be extended every 10 seconds. With thermal stability, Taq DNA polymerase will not be inactivated at temperature above 90°C, therefo......2015-09-28

Currenlty, there are two kinds of ELISA kits for target protein detection on the market, one of them uses double antibody sandwich method, and the other one uses competitive inhibition method. In the operation processes, both of these two ELISA kits need about 3 to 5 hours, various kinds of reagents preparation, and many steps and incubations. So, these factors will lead to higher posibility of error and pollution, and consequently, the experiment requires higher demand of skills for researchers. In order to increase the experimental efficiency, and decrease potential error, we had developed a series of instant ELISA kits. This kind of kits applied antibodies which are more sensitive ......2015-09-28

Library Introduction cDNA library (Complementary DNA Library) is a mixture of various contructs, which contains different cDNA fragments which is created from a mature mRNA from eukaryotic cells with the use of an enzyme known as reverse transcriptase. Those constructs are easy to express various proteins, unlike genome, therefore target genes can be screened from cDNA library and expressed directly. By constructing cDNA expression library, the probe of molecular marker linkage map can be provided, more importantly, it can be used to isolate full-length target gene and study its biological function. Thus, cDNA library has special advantage in the study of certain cell expression stat......2015-09-25

With more widely application of antibiotics in clinical practice and discovery of new viruses such as SARS and H7N9, new problems have cropped up. Due to untypical clinical symptoms and limited experience of doctors, diagnosing diseases with similar symptoms becomes difficult. Especially for bacterial infections and non-bacterial infections, their treatments are totally different. Once misdiagnosis occurs, the best treatment time might be missed. That would lead to serious consequences such as disease deterioration. Therefore, how should we judge whether it is bacterial infection or non-bacterial infection? Researchers found that, PCT (procalcitonin) is a kind of special protein, its ......2015-09-23

The aim of molecular cloning vector construction is to obtain target proteins. E. coli, yeast, insect, plant and mammalian cell expression systems are common expression systems. E. Coli expression system is the most popular one with many advantages such as the identified genetic background, simple operation, high yield, low price and so on. Separation and purification of recombinant protein is the base of developing and producing proteins, antibodies and Elisa kits.Common purification methods include ion exchange chromatography (IEX),Hydrophobic interaction chromatography (HIC), size exclusion chromatography (SEC) and electrophoresis, etc. The purity, reproducibility, stability and cos......2015-09-07

Autophagy, proposed by Ashford and Porter in 1962 after they found the "eat themselves" phenomenon within cells, refers to part of cytoplasm from no ribosome attached district of rough endoplasmic reticulum, which was embed by bilayer membrane. The cytoplasm and the organelles, and protein and the other components need degradation formed autophagosome. The autophagosome and lysosome merged and formed autophagosome-lysosome complex, the complex will degrade all the embed content. In this way, the metabolism demand of the cells will be met and some organelles will be updated . Currently autophagy is one of the most hottest topics in life science area following apoptosis ,accord......2015-09-07