Keratin 12 (KRT12)

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CK12; K12; Cytokeratin 12; Meesmann Corneal Dystrophy; Keratin, type I cytoskeletal 12

Keratin 12 (KRT12)

Keratin 12 is a keratin found expressed in corneal epithelia. Mutations in the gene encoding this protein lead to Meesmann corneal dystrophy. To elucidate the function of keratin 12, Kao et al. (1996) created knockout mice lacking the Krt1.12 gene by gene targeting techniques. The heterozygous mice appeared normal. Homozygous mice developed normally and suffered mild corneal epithelial erosion. The corneal epithelia were fragile and could be removed by gentle rubbing of the eyes or brushing. The corneal epithelium of the homozygotes did not express keratin 12 as judged by immunohistochemistry, Western immunoblot analysis with epitope-specific anti-keratin 12 antibodies, Northern hybridization, and in situ hybridization with an antisense keratin 12 riboprobe.

Organism species: Homo sapiens (Human)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Keratin 12 (KRT12)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Keratin 12 (KRT12)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Keratin 12 (KRT12)Polyclonal Antibody Customized Service Offer
Assay KitsSEA535HuELISA Kit for Keratin 12 (KRT12)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
Proteinsn/aRecombinant Keratin 12 (KRT12)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Keratin 12 (KRT12)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Keratin 12 (KRT12)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Keratin 12 (KRT12)CLIA Kit Customized Service Offer
n/aELISA Kit for Keratin 12 (KRT12)ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO.PRODUCT NAMEAPPLICATIONS
ProteinsRPA535Ra01Recombinant Keratin 12 (KRT12)SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
AntibodiesPAA535Ra01Polyclonal Antibody to Keratin 12 (KRT12)WB, ICC, IHC-P, IHC-F, ELISA
LAA535Ra71Biotin-Linked Polyclonal Antibody to Keratin 12 (KRT12)IHC;WB;ELISA
Assay Kitsn/aCLIA Kit for Keratin 12 (KRT12)CLIA Kit Customized Service Offer
n/aELISA Kit for Keratin 12 (KRT12)ELISA Kit Customized Service Offer
  1. "A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA."Invest. Ophthalmol. Vis. Sci. 37:1800-1809(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Isolation and chromosomal localization of a cornea-specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy."Am. J. Hum. Genet. 61:1268-1275(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Molecular genetics of Meesmann's corneal dystrophy: ancestral and novel mutations in keratin 12 (K12) and complete sequence of the human KRT12 gene."Exp. Eye Res. 70:41-49(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  7. "Mutations in cornea-specific keratin K3 or K12 genes cause Meesmann's corneal dystrophy."Nat. Genet. 16:184-187(1997) [PubMed] [Europe PMC] [Abstract]
  8. "A novel keratin 12 mutation in a German kindred with Meesmann's corneal dystrophy."Br. J. Ophthalmol. 84:527-530(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Heterozygous Ala137Pro mutation in keratin 12 gene found in Japanese with Meesmann's corneal dystrophy."Jpn. J. Ophthalmol. 46:673-674(2002) [PubMed] [Europe PMC] [Abstract]
  10. "A novel arginine substitution mutation in 1A domain and a novel 27 bp insertion mutation in 2B domain of keratin 12 gene associated with Meesmann's corneal dystrophy."Br. J. Ophthalmol. 88:752-756(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Novel mutations in the helix termination motif of keratin 3 and keratin 12 in 2 Taiwanese families with Meesmann corneal dystrophy."Cornea 24:928-932(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Meesmann corneal dystrophy (MECD): report of 2 families and a novel mutation in the cornea specific keratin 12 (KRT12) gene."Ophthalmic Genet. 26:169-173(2005) [PubMed] [Europe PMC] [Abstract]
  13. "A novel mutation of the Keratin 12 gene responsible for a severe phenotype of Meesmann's corneal dystrophy."Mol. Vis. 13:975-980(2007) [PubMed] [Europe PMC] [Abstract]
  14. "A novel mutation as the basis for asymptomatic meesmann dystrophy in a Danish family."Cornea 27:100-102(2008) [PubMed] [Europe PMC] [Abstract]
  15. "A novel mutation in the cornea-specific keratin 12 gene in Meesmann corneal dystrophy."Jpn. J. Ophthalmol. 52:224-226(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Identification of a novel mutation in the cornea specific keratin 12 gene causing Meesmann's corneal dystrophy in a German family."Mol. Vis. 16:954-960(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Severe Meesmann's epithelial corneal dystrophy phenotype due to a missense mutation in the helix-initiation motif of keratin 12."Eye 27:367-373(2013) [PubMed] [Europe PMC] [Abstract]
  18. "KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy."Am. J. Ophthalmol. 157:93-102(2014) [PubMed] [Europe PMC] [Abstract]