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Cytokeratin 12 (CK12)

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KRT12; K12; Keratin 12; Meesmann Corneal Dystrophy; Keratin, type I cytoskeletal 12

Cytokeratin 12 (CK12)
CCC A535
HGNC 6414
MGI 96687
PubMed 16431949
RGD 1304805
UniProt Q99456
Wiki Keratin_12

Keratin 12 is a keratin found expressed in corneal epithelia. Mutations in the gene encoding this protein lead to Meesmann corneal dystrophy. To elucidate the function of keratin 12, Kao et al. (1996) created knockout mice lacking the Krt1.12 gene by gene targeting techniques. The heterozygous mice appeared normal. Homozygous mice developed normally and suffered mild corneal epithelial erosion. The corneal epithelia were fragile and could be removed by gentle rubbing of the eyes or brushing. The corneal epithelium of the homozygotes did not express keratin 12 as judged by immunohistochemistry, Western immunoblot analysis with epitope-specific anti-keratin 12 antibodies, Northern hybridization, and in situ hybridization with an antisense keratin 12 riboprobe.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cytokeratin 12 (CK12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cytokeratin 12 (CK12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cytokeratin 12 (CK12) Polyclonal Antibody Customized Service Offer
Assay Kits SEA535Hu ELISA Kit for Cytokeratin 12 (CK12) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins n/a Recombinant Cytokeratin 12 (CK12) Recombinant Protein Customized Service Offer
Antibodies n/a Monoclonal Antibody to Cytokeratin 12 (CK12) Monoclonal Antibody Customized Service Offer
n/a Polyclonal Antibody to Cytokeratin 12 (CK12) Polyclonal Antibody Customized Service Offer
Assay Kits n/a CLIA Kit for Cytokeratin 12 (CK12) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytokeratin 12 (CK12) ELISA Kit Customized Service Offer

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA535Ra01 Recombinant Cytokeratin 12 (CK12) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA535Ra01 Polyclonal Antibody to Cytokeratin 12 (CK12) WB; IHC; ICC; IP.
LAA535Ra71 Biotin-Linked Polyclonal Antibody to Cytokeratin 12 (CK12) WB; IHC; ICC.
Assay Kits n/a CLIA Kit for Cytokeratin 12 (CK12) CLIA Kit Customized Service Offer
n/a ELISA Kit for Cytokeratin 12 (CK12) ELISA Kit Customized Service Offer
  1. "A gene expression profile of human corneal epithelium and the isolation of human keratin 12 cDNA."Invest. Ophthalmol. Vis. Sci. 37:1800-1809(1996) [PubMed] [Europe PMC] [Abstract]
  2. "Isolation and chromosomal localization of a cornea-specific human keratin 12 gene and detection of four mutations in Meesmann corneal epithelial dystrophy."Am. J. Hum. Genet. 61:1268-1275(1997) [PubMed] [Europe PMC] [Abstract]
  3. "Molecular genetics of Meesmann's corneal dystrophy: ancestral and novel mutations in keratin 12 (K12) and complete sequence of the human KRT12 gene."Exp. Eye Res. 70:41-49(2000) [PubMed] [Europe PMC] [Abstract]
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  5. "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides."Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract]
  6. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  7. "Mutations in cornea-specific keratin K3 or K12 genes cause Meesmann's corneal dystrophy."Nat. Genet. 16:184-187(1997) [PubMed] [Europe PMC] [Abstract]
  8. "A novel keratin 12 mutation in a German kindred with Meesmann's corneal dystrophy."Br. J. Ophthalmol. 84:527-530(2000) [PubMed] [Europe PMC] [Abstract]
  9. "Heterozygous Ala137Pro mutation in keratin 12 gene found in Japanese with Meesmann's corneal dystrophy."Jpn. J. Ophthalmol. 46:673-674(2002) [PubMed] [Europe PMC] [Abstract]
  10. "A novel arginine substitution mutation in 1A domain and a novel 27 bp insertion mutation in 2B domain of keratin 12 gene associated with Meesmann's corneal dystrophy."Br. J. Ophthalmol. 88:752-756(2004) [PubMed] [Europe PMC] [Abstract]
  11. "Novel mutations in the helix termination motif of keratin 3 and keratin 12 in 2 Taiwanese families with Meesmann corneal dystrophy."Cornea 24:928-932(2005) [PubMed] [Europe PMC] [Abstract]
  12. "Meesmann corneal dystrophy (MECD): report of 2 families and a novel mutation in the cornea specific keratin 12 (KRT12) gene."Ophthalmic Genet. 26:169-173(2005) [PubMed] [Europe PMC] [Abstract]
  13. "A novel mutation of the Keratin 12 gene responsible for a severe phenotype of Meesmann's corneal dystrophy."Mol. Vis. 13:975-980(2007) [PubMed] [Europe PMC] [Abstract]
  14. "A novel mutation as the basis for asymptomatic meesmann dystrophy in a Danish family."Cornea 27:100-102(2008) [PubMed] [Europe PMC] [Abstract]
  15. "A novel mutation in the cornea-specific keratin 12 gene in Meesmann corneal dystrophy."Jpn. J. Ophthalmol. 52:224-226(2008) [PubMed] [Europe PMC] [Abstract]
  16. "Identification of a novel mutation in the cornea specific keratin 12 gene causing Meesmann's corneal dystrophy in a German family."Mol. Vis. 16:954-960(2010) [PubMed] [Europe PMC] [Abstract]
  17. "Severe Meesmann's epithelial corneal dystrophy phenotype due to a missense mutation in the helix-initiation motif of keratin 12."Eye 27:367-373(2013) [PubMed] [Europe PMC] [Abstract]
  18. "KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy."Am. J. Ophthalmol. 157:93-102(2014) [PubMed] [Europe PMC] [Abstract]