Packages (Simulation)

Polyclonal Antibody to Cytokeratin 12 (CK12)

Image (I)

IHC Images
DAB staining on IHC-P;
Sample: Rabbit Eye Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat CK12 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

Image (II)

Western Blot; Sample: Lane1: Rat eyes lysate; Lane2: Mouse eyes lysate Primary Ab: 2µg/ml Rabbit Anti-Rat KRT12 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
Western Blot; Sample: Lane1: Porcine eyes lysate; Lane2: WERI-Rb-1 cell lysate
Primary Ab: 1µg/ml Rabbit Anti-Rat KRT12 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)

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Instruction Manual

Polyclonal-Antibody-to-Cytokeratin-12-(CK12)-PAA535Ra01.pdf

Certificate

Polyclonal Antibody to Cytokeratin 12 (CK12)

KRT12; K12; Keratin 12; Meesmann Corneal Dystrophy; Keratin, type I cytoskeletal 12

Product No.PAA535Ra01
Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- All
- Human
- Mouse
- Rat
- Cavia
- Rabbit
- Simian
- Canine
- Porcine
- Bovine
- Caprine
- Multi-species
- Pan-species
- Others
SourcePolyclonal antibody preparation
HostRabbit
Potencyn/a
Ig Type IgG
PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
LabelNone
Immunogen RPA535Ra01-Recombinant Cytokeratin 12 (CK12)
Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
TraitsLiquid
Concentration0.5mg/ml
Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse), Sus scrofa; Porcine (Pig)
ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
DownloadInstruction Manual
UOM 20µl100µl 200µl 1ml 10ml
FOB US$ 103 239 US$ 239 US$ 342 US$ 855 US$ 3420
For more details, please contact local distributors!

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against CK12. It has been selected for its ability to recognize CK12 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Catalog No.	Related products for research use of Rattus norvegicus (Rat) Organism species	Applications (RESEARCH USE ONLY!)
RPA535Ra01	Recombinant Cytokeratin 12 (CK12)	Positive Control; Immunogen; SDS-PAGE; WB.
PAA535Ra01	Polyclonal Antibody to Cytokeratin 12 (CK12)	WB; IHC; ICC; IP.
LAA535Ra71	Biotin-Linked Polyclonal Antibody to Cytokeratin 12 (CK12)	WB; IHC; ICC.