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Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

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PEPCK1; PEPCKC; Cytosolic Phosphoenolpyruvate Carboxykinase; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)
CCC A936
HGNC 8724
MGI 97501
PubMed 14764811
RGD 3267
UniProt P35558
Wiki PCK1

Phosphoenolpyruvate carboxykinase is an enzyme used in the natural process of gluconeogenesis. It converts oxaloacetate into phosphoenolpyruvate and carbon dioxide.

Phosphoenolpyruvate carboxykinase 1 (soluble), also known as PCK1, is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Organism species: Homo sapiens (Human)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA936Hu01 Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA936Hu01 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB
MAA936Hu22 Monoclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB; IHC; ICC; IP.
Assay Kits SEA936Hu ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Mus musculus (Mouse)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA936Mu01 Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Positive Control; Immunogen; SDS-PAGE; WB.
RPA936Mu02 Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA936Mu01 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB; IHC; ICC; IP.
PAA936Mu02 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB; IHC; ICC; IP.
Assay Kits SEA936Mu ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

CATALOG NO. PRODUCT NAME APPLICATIONS
Proteins RPA936Ra01 Recombinant Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Positive Control; Immunogen; SDS-PAGE; WB.
Antibodies PAA936Ra01 Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB; IHC; ICC; IP.
MAA936Ra21 Monoclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) WB; IHC; ICC; IP.
Assay Kits SEA936Ra ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1) Enzyme-linked immunosorbent assay for Antigen Detection.
  1. "cDNA sequence and localization of polymorphic human cytosolic phosphoenolpyruvate carboxykinase gene (PCK1) to chromosome 20, band q13.31: PCK1 is not tightly linked to maturity-onset diabetes of the young."Hum. Mol. Genet. 2:1-4(1993) [PubMed] [Europe PMC] [Abstract]
  2. "Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20."Genomics 16:698-706(1993) [PubMed] [Europe PMC] [Abstract]
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs." Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
  4. "The DNA sequence and comparative analysis of human chromosome 20." Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract]
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  6. "Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter."Biochim. Biophys. Acta 1264:284-288(1995) [PubMed] [Europe PMC] [Abstract]
  7. "Regulation of phosphoenolpyruvate carboxykinase (GTP) gene transcription."Mol. Cell. Biochem. 104:89-100(1991) [PubMed] [Europe PMC] [Abstract]
  8. "Regulation of cellular metabolism by protein lysine acetylation." Science 327:1000-1004(2010) [PubMed] [Europe PMC] [Abstract]
  9. "Acetylation regulates gluconeogenesis by promoting PEPCK1 degradation via recruiting the UBR5 ubiquitin ligase."Mol. Cell 43:33-44(2011) [PubMed] [Europe PMC] [Abstract]
  10. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  11. "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site."J. Mol. Biol. 316:257-264(2002) [PubMed] [Europe PMC] [Abstract]