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Polyclonal Antibody to Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)

PEPCK1; PEPCKC; Cytosolic Phosphoenolpyruvate Carboxykinase; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against PCK1. It has been selected for its ability to recognize PCK1 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Magazine Citations
Endocrinology In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland Pubmed: 24564399
Cellular Physiology and Biochemistry Overexpression of PCK1 Gene Antagonizes Hepatocellular Carcinoma Through the Activation of Gluconeogenesis and Suppression of Glycolysis Pathways Pubmed:29768256
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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