Proteinase 3 (PR3)


MBN; PRTN3; ACPA; AGP7; C-ANCA; MBT; P29; PRK; Myeloblastin; Serine Proteinase,Neutrophil,Wegener Granulomatosis Autoantigen; Protease K; Proteinase K; Endopeptidase K

Proteinase 3 (PR3)

PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown but in human neutrophils proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the epitope of anti-neutrophil cytoplasmic antibodies (ANCAs) of the cANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease Wegener's granulomatosis.

Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. Addition of proteinase K to nucleic acid preparations rapidly inactivates nucleases that might otherwise degrade the DNA or RNA during purification.

Organism species: Homo sapiens (Human)

ProteinsRPB434Hu01Recombinant Proteinase 3 (PR3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB434Hu01Polyclonal Antibody to Proteinase 3 (PR3)WB, ICC, IHC-P, IHC-F, ELISA
MAB434Hu22Monoclonal Antibody to Proteinase 3 (PR3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB434HuELISA Kit for Proteinase 3 (PR3)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Mus musculus (Mouse)

ProteinsRPB434Mu01Recombinant Proteinase 3 (PR3)SDS-PAGE; WB; ELISA; IP.
RPB434Mu02Recombinant Proteinase 3 (PR3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB434Mu01Polyclonal Antibody to Proteinase 3 (PR3)WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
LAB434Mu71Biotin-Linked Polyclonal Antibody to Proteinase 3 (PR3)IHC;WB;ELISA
PAB434Mu02Polyclonal Antibody to Proteinase 3 (PR3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB434MuELISA Kit for Proteinase 3 (PR3)Enzyme-linked immunosorbent assay for Antigen Detection

Organism species: Rattus norvegicus (Rat)

ProteinsRPB434Ra01Recombinant Proteinase 3 (PR3)SDS-PAGE; WB; ELISA; IP.
AntibodiesPAB434Ra01Polyclonal Antibody to Proteinase 3 (PR3)WB, ICC, IHC-P, IHC-F, ELISA
LAB434Ra71Biotin-Linked Polyclonal Antibody to Proteinase 3 (PR3)IHC;WB;ELISA
MAB434Ra21Monoclonal Antibody to Proteinase 3 (PR3)WB, ICC, IHC-P, IHC-F, ELISA
Assay KitsSEB434RaELISA Kit for Proteinase 3 (PR3)Enzyme-linked immunosorbent assay for Antigen Detection
  1. "Wegener autoantigen and myeloblastin are encoded by a single mRNA."Proc. Natl. Acad. Sci. U.S.A. 88:9253-9256(1991) [PubMed] [Europe PMC] [Abstract]
  2. "The DNA sequence and biology of human chromosome 19." Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Three human elastase-like genes coordinately expressed in the myelomonocyte lineage are organized as a single genetic locus on 19pter."Proc. Natl. Acad. Sci. U.S.A. 89:8215-8219(1992) [PubMed] [Europe PMC] [Abstract]
  5. "Structure, chromosomal assignment, and expression of the gene for proteinase-3. The Wegener's granulomatosis autoantigen."J. Biol. Chem. 267:21193-21199(1992) [PubMed] [Europe PMC] [Abstract]
  6. "Donor-recipient polymorphism of the proteinase 3 gene: a potential target for T-cell alloresponses to myeloid leukemia."J. Immunother. 22:1-6(1999) [PubMed] [Europe PMC] [Abstract]
  7. "Wegener's autoantigen and leukemia."Blood 77:1398-1399(1991) [PubMed] [Europe PMC] [Abstract]
  8. "Cloning of cDNA for proteinase 3: a serine protease, antibiotic, and autoantigen from human neutrophils."J. Exp. Med. 172:1709-1715(1990) [PubMed] [Europe PMC] [Abstract]
  9. "Down-regulation of a serine protease, myeloblastin, causes growth arrest and differentiation of promyelocytic leukemia cells."Cell 59:959-968(1989) [PubMed] [Europe PMC] [Abstract]
  10. "Wegener's autoantigen decoded."Nature 346:520-520(1990) [PubMed] [Europe PMC] [Abstract]
  11. "Characterization of proteinase-3 (PR-3), a neutrophil serine proteinase. Structural and functional properties."J. Biol. Chem. 266:9540-9548(1991) [PubMed] [Europe PMC] [Abstract]
  12. "Characterization of two azurphil granule proteases with active-site homology to neutrophil elastase."J. Biol. Chem. 265:2038-2041(1990) [PubMed] [Europe PMC] [Abstract]
  13. "Monoclonal antibodies specific for neutrophil proteinase 4. Production and use for isolation of the enzyme."Biol. Chem. Hoppe-Seyler 371:549-555(1990) [PubMed] [Europe PMC] [Abstract]
  14. "The relation of 29 kD C-ANCA antigen to proteinase 3."APMIS Suppl. 19:26-27(1990) [PubMed] [Europe PMC] [Abstract]
  15. "Wegener's granulomatosis autoantigen is a novel neutrophil serine proteinase."Blood 74:1888-1893(1989) [PubMed] [Europe PMC] [Abstract]
  16. "Antibiotic proteins of human polymorphonuclear leukocytes."Proc. Natl. Acad. Sci. U.S.A. 86:5610-5614(1989) [PubMed] [Europe PMC] [Abstract]
  17. "Use of proteinase 3 purified by reverse phase HPLC to detect autoantibodies in systemic vasculitis."J. Immunol. Methods 180:25-33(1995) [PubMed] [Europe PMC] [Abstract]
  18. "Anti-neutrophil cytoplasm antibodies in Wegener's granulomatosis recognize an elastinolytic enzyme."J. Exp. Med. 171:357-362(1990) [PubMed] [Europe PMC] [Abstract]
  19. "A novel form of dipeptidylpeptidase IV found in human serum. Isolation, characterization, and comparison with T lymphocyte membrane dipeptidylpeptidase IV (CD26)."J. Biol. Chem. 270:14107-14114(1995) [PubMed] [Europe PMC] [Abstract]
  20. "Identity of Wegener's autoantigen (p29) with proteinase 3 and myeloblastin."Blood 76:2162-2162(1990) [PubMed] [Europe PMC] [Abstract]
  21. "Initial characterization of the human central proteome."BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
  22. "The crystal structure of PR3, a neutrophil serine proteinase antigen of Wegener's granulomatosis antibodies."J. Mol. Biol. 261:267-278(1996) [PubMed] [Europe PMC] [Abstract]