Phospholamban (PLN)

Phospholamban is a 52 amino acid integral membrane protein that regulates the Ca2 pump in cardiac muscle and skeletal muscle cells. When phospholamban is phosphorylated by protein kinase A (PKA) its ability to to inhibit the sarcoplasmic reticulum calcium pump (SERCA) is lost. Thus, activators of PKA, such as the beta-adrenergic agonist epinephrine (released by sympathetic stimulation) may enhance the rate of cardiac myocyte relaxation.

Additionally, since SERCA is more active, the next action potential will cause an increased release of calcium resulting in increased contraction (positive inotropic effect). When phospholamban is not phosphorylated, such as when PKA is inactive, it can interact with and inhibit SERCA.

Organism species: Homo sapiens (Human)

ProteinsRPB884Hu01Recombinant Phospholamban (PLN)Positive Control; Immunogen; SDS-PAGE; WB.
AntibodiesPAB884Hu01Polyclonal Antibody to Phospholamban (PLN)WB; IHC; ICC; IP.
Assay Kitsn/aCLIA Kit for Phospholamban (PLN)CLIA Kit Customized Service Offer
n/aELISA Kit for Phospholamban (PLN)ELISA Kit Customized Service Offer

Organism species: Mus musculus (Mouse)

ProteinsRPB884Mu01Recombinant Phospholamban (PLN)Positive Control; Immunogen; SDS-PAGE; WB.
RPB884Mu02Recombinant Phospholamban (PLN)Positive Control; Immunogen; SDS-PAGE; WB.
AntibodiesPAB884Mu01Polyclonal Antibody to Phospholamban (PLN)WB; IHC; ICC; IP.
PAB884Mu02Polyclonal Antibody to Phospholamban (PLN)WB; IHC; ICC; IP.
Assay KitsSEB884MuELISA Kit for Phospholamban (PLN)Enzyme-linked immunosorbent assay for Antigen Detection.

Organism species: Rattus norvegicus (Rat)

Proteinsn/aRecombinant Phospholamban (PLN)Recombinant Protein Customized Service Offer
Antibodiesn/aMonoclonal Antibody to Phospholamban (PLN)Monoclonal Antibody Customized Service Offer
n/aPolyclonal Antibody to Phospholamban (PLN)Polyclonal Antibody Customized Service Offer
Assay Kitsn/aCLIA Kit for Phospholamban (PLN)CLIA Kit Customized Service Offer
n/aELISA Kit for Phospholamban (PLN)ELISA Kit Customized Service Offer
  1. "Structure of the rabbit phospholamban gene, cloning of the human cDNA, and assignment of the gene to human chromosome 6."J. Biol. Chem. 266:11669-11675(1991) [PubMed] [Europe PMC] [Abstract]
  2. "The human phospholamban gene: structure and expression."J. Mol. Cell. Cardiol. 31:679-692(1999) [PubMed] [Europe PMC] [Abstract]
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
  4. "Mutation of the phospholamban promoter associated with hypertrophic cardiomyopathy."Biochem. Biophys. Res. Commun. 304:1-4(2003) [PubMed] [Europe PMC] [Abstract]
  5. "Myotonic dystrophy protein kinase phosphorylates phospholamban and regulates calcium uptake in cardiomyocyte sarcoplasmic reticulum."J. Biol. Chem. 280:8016-8021(2005) [PubMed] [Europe PMC] [Abstract]
  6. "Ca2+-calmodulin-dependent protein kinase expression and signalling in skeletal muscle during exercise."J. Physiol. (Lond.) 574:889-903(2006) [PubMed] [Europe PMC] [Abstract]
  7. "Phospholamban interacts with HAX-1, a mitochondrial protein with anti-apoptotic function."J. Mol. Biol. 367:65-79(2007) [PubMed] [Europe PMC] [Abstract]
  8. "Lethal, hereditary mutants of phospholamban elude phosphorylation by protein kinase A."J. Biol. Chem. 287:26596-26605(2012) [PubMed] [Europe PMC] [Abstract]
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
  10. "Solution structure of the cytoplasmic domain of phospholamban: phosphorylation leads to a local perturbation in secondary structure."Biochemistry 34:7603-7613(1995) [PubMed] [Europe PMC] [Abstract]
  11. "Computational searching and mutagenesis suggest a structure for the pentameric transmembrane domain of phospholamban."Nat. Struct. Biol. 2:154-162(1995) [PubMed] [Europe PMC] [Abstract]
  12. "Using experimental information to produce a model of the transmembrane domain of the ion channel phospholamban."Biophys. J. 74:1203-1214(1998) [PubMed] [Europe PMC] [Abstract]
  13. "The structure of phospholamban pentamer reveals a channel-like architecture in membranes."Proc. Natl. Acad. Sci. U.S.A. 102:10870-10875(2005) [PubMed] [Europe PMC] [Abstract]
  14. "Structure determination of symmetric homo-oligomers by a complete search of symmetry configuration space, using NMR restraints and van der Waals packing."Proteins 65:203-219(2006) [PubMed] [Europe PMC] [Abstract]
  15. "Dilated cardiomyopathy and heart failure caused by a mutation in phospholamban."Science 299:1410-1413(2003) [PubMed] [Europe PMC] [Abstract]
  16. "A mutation in the human phospholamban gene, deleting arginine 14, results in lethal, hereditary cardiomyopathy."Proc. Natl. Acad. Sci. U.S.A. 103:1388-1393(2006) [PubMed] [Europe PMC] [Abstract]
  17. "Mutations in the human phospholamban gene in patients with heart failure."Am. Heart J. 162:1088-1095(2011) [PubMed] [Europe PMC] [Abstract]
  18. "Hydrophobic imbalance in the cytoplasmic domain of phospholamban is a determinant for lethal dilated cardiomyopathy."J. Biol. Chem. 287:16521-16529(2012) [PubMed] [Europe PMC] [Abstract]