ELISA Kit for Anti-S100 Calcium Binding Protein Antibody (Anti-S100)

S100-A1; S100A1; S100-Alpha; S-100 Protein; S-100 protein subunit alpha; S-100 protein alpha chain

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-S100 Calcium Binding Protein Antibody (Anti-S100).
No significant cross-reactivity or interference between Anti-S100 Calcium Binding Protein Antibody (Anti-S100) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Anti-S100 Calcium Binding Protein Antibody (Anti-S100) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-S100 Calcium Binding Protein Antibody (Anti-S100) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-105 101
EDTA plasma(n=5) 85-101 91
heparin plasma(n=5) 93-101 97

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-S100 Calcium Binding Protein Antibody (Anti-S100) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-S100 Calcium Binding Protein Antibody (Anti-S100) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-S100 Calcium Binding Protein Antibody (Anti-S100) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-97% 78-94% 80-105% 93-105%
EDTA plasma(n=5) 87-95% 78-96% 98-105% 79-90%
heparin plasma(n=5) 94-101% 95-102% 85-93% 78-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Brain Research Vasoactive intestinal peptide protects against ischemic brain damage induced by focal cerebral ischemia in rats ScienceDirect: S0006899311008663
J Exp Neurosci A New Technique for Collection of Cerebrospinal Fluid in Rat Pups PubMed: 26056488
PLoS One Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine pubmed:27501393
brain research Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. pubmed:28238669
КиберЛенинка Levels of neurotropic autoantibodies in patients with schizophrenia article:n
Biomed Research International Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336
Metabolic Brain Disease Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms Pubmed: 30421246
ВОЗМОЖНОСТЬ ПРОГНОЗИРОВАНИЯ ОСТРОЙ НЕСПЕЦИФИЧЕСКОЙ БОЛИ В НИЖНЕЙ ЧАСТИ СПИНЫ С ИСПОЛЬЗОВАНИЕМ СЫВОРОТОЧНЫХ …
ВЛИЯНИЕ ХРОНИЧЕСКОГО ВВЕДЕНИЯ НЕЙРОЛЕПТИКОВ НА УРОВНИ НЕЙРОСПЕЦИФИЧЕСКИХ АУТОАНТИТЕЛ В КРОВИ У КРЫС
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA012Hu01 Recombinant S100 Calcium Binding Protein (S100) Positive Control; Immunogen; SDS-PAGE; WB.
PAA012Hu01 Polyclonal Antibody to S100 Calcium Binding Protein (S100) WB; ICC; IHC-P; IHC-F; ELISA.
LAA012Hu71 Biotin-Linked Polyclonal Antibody to S100 Calcium Binding Protein (S100) WB; IHC; ICC.
LAA012Hu81 FITC-Linked Polyclonal Antibody to S100 Calcium Binding Protein (S100) WB; IHC; ICC; IF.
MAA012Hu21 Monoclonal Antibody to S100 Calcium Binding Protein (S100) WB; IHC; ICC; IP.
MAA012Hu22 Monoclonal Antibody to S100 Calcium Binding Protein (S100) WB; IHC; ICC; IP.
AEA012Hu ELISA Kit for Anti-S100 Calcium Binding Protein Antibody (Anti-S100) Enzyme-linked immunosorbent assay for Antibody Detection.
SEA012Hu ELISA Kit for S100 Calcium Binding Protein (S100) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA012Hu CLIA Kit for S100 Calcium Binding Protein (S100) Chemiluminescent immunoassay for Antigen Detection.
PSA012Hu01 Antibody Pair for S100 Calcium Binding Protein (S100) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA012Hu01 ELISA Kit DIY Materials for S100 Calcium Binding Protein (S100) Main materials for "Do It (ELISA Kit) Yourself".