ELISA Kit for Anti-Thyroglobulin Antibody (Anti-TG)



This assay has high sensitivity and excellent specificity for detection of Anti-Thyroglobulin Antibody (Anti-TG).
No significant cross-reactivity or interference between Anti-Thyroglobulin Antibody (Anti-TG) and analogues was observed.


Matrices listed below were spiked with certain level of Anti-Thyroglobulin Antibody (Anti-TG) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Thyroglobulin Antibody (Anti-TG) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-99 92
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 78-88 82


Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Thyroglobulin Antibody (Anti-TG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Thyroglobulin Antibody (Anti-TG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Thyroglobulin Antibody (Anti-TG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 80-89% 85-92% 80-94% 91-98%
EDTA plasma(n=5) 79-93% 80-97% 91-98% 87-105%
heparin plasma(n=5) 97-104% 94-101% 80-96% 86-94%


The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.



Magazine Citations
Thyroid. Iodine Storage and Metabolism of Mild to Moderate Iodine-Deficient Pregnant Rats. pubmed:28358234
Lipids in Health and Disease Heat shock protein 70 promotes lipogenesis in HepG2 cells Pubmed:29631603
Available at SSRN 3335365  Evaluating the Platelet Activation Related to the Degradation of Biomaterials by Scheme of Molecular Markers
journal of thoracic and cardiovascular surgery Evaluating Platelet Activation Related to the Degradation of Biomaterials Using Molecular Markers Pubmed: 32812629
Marine Drugs The Inhibitory Effect of Protamine on Platelets is Attenuated by Heparin without Inducing Thrombocytopenia in Rodents Pubmed: 31533230
ACS Omega. Purification and Characterization of Novel Collagen Peptides against Platelet Aggregation and Thrombosis from Salmo salar Pubmed: 32832753
Evid Based Complement Alternat Med Effect of Acupoint Embedding on Serum Leptin and Hypothalamus Leptin Receptor Expression in Rats with Simple Obesity 34659432
Antioxidants Antioxidant Effect of Tyr-Ala Extracted from Zein on INS-1 Cells and Type 2 Diabetes High-Fat-Diet-Induced Mice Pubmed:35740008
Journal of Food Science Tripeptide Hyp‐Asp‐Gly from collagen peptides inhibited platelet activation via regulation of PI3K/Akt‐MAPK/ERK1/2 signaling pathway Pubmed:35703476
Catalog No. Related products for research use of Rattus norvegicus (Rat) Organism species Applications (RESEARCH USE ONLY!)
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