ELISA Kit for Anti-Complement 1q Antibody (Anti-C1q)

C1-q; Autoantigen Clq

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Complement 1q Antibody (Anti-C1q).
No significant cross-reactivity or interference between Anti-Complement 1q Antibody (Anti-C1q) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Anti-Complement 1q Antibody (Anti-C1q) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Complement 1q Antibody (Anti-C1q) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-103 89
EDTA plasma(n=5) 78-101 82
heparin plasma(n=5) 91-98 95

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Complement 1q Antibody (Anti-C1q) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Complement 1q Antibody (Anti-C1q) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-97% 92-101% 79-93% 98-105%
EDTA plasma(n=5) 94-101% 97-105% 98-105% 83-94%
heparin plasma(n=5) 82-89% 85-97% 95-104% 79-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Journal of Clinical Immunology Complement activation contributes to the injury and outcome of kidney in human anti-glomerular basement membrane disease. PubMed: 22941511
Fish and Shellfish Immunology Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity Pubmed: 23085472
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
Archives of Gynecology and Obstetrics The role of complement components C1q, MBL and C1 inhibitor in pathogenesis of endometriosis Pubmed:29572748
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
PLoS One Complement activation profile of patients with primary focal segmental glomerulosclerosis Pubmed: 32569286
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
PAA747Hu01 Polyclonal Antibody to Complement 1q (C1q) WB; IHC; ICC; IP.
LAA747Hu71 Biotin-Linked Polyclonal Antibody to Complement 1q (C1q) WB; IHC; ICC.
SEA747Hu ELISA Kit for Complement 1q (C1q) Enzyme-linked immunosorbent assay for Antigen Detection.
AEA747Hu ELISA Kit for Anti-Complement 1q Antibody (Anti-C1q) Enzyme-linked immunosorbent assay for Antibody Detection.
KSA747Hu01 ELISA Kit DIY Materials for Complement 1q (C1q) Main materials for "Do It (ELISA Kit) Yourself".