ELISA Kit for Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb)

CG-B; CGB3; hCGB; HCG; CGb5; CGb7; CGb8; Choriogonadotropin subunit beta

Specificity

This assay has high sensitivity and excellent specificity for detection of Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb).
No significant cross-reactivity or interference between Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 97
EDTA plasma(n=5) 92-99 95
heparin plasma(n=5) 94-103 99

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 84-92% 84-94% 82-99% 98-105%
EDTA plasma(n=5) 83-90% 99-105% 90-104% 80-89%
heparin plasma(n=5) 84-92% 95-105% 78-91% 92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
临床输血与检验 联合检测血清血管内皮生长因子和β-HCG对早期异位妊娠的诊断意义 1671-2587
Biomarkers Regulation of circulating chromogranin B levels in heart failure Pubmed:29098879
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPD215Hu01 Recombinant Chorionic Gonadotropin Beta Polypeptide (CGb) Positive Control; Immunogen; SDS-PAGE; WB.
PAD215Hu01 Polyclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
MAD215Hu21 Monoclonal Antibody to Chorionic Gonadotropin Beta Polypeptide (CGb) WB; IHC; ICC; IP.
SED215Hu ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Enzyme-linked immunosorbent assay for Antigen Detection.
CED215Hu ELISA Kit for Chorionic Gonadotropin Beta Polypeptide (CGb) Enzyme-linked immunosorbent assay for Antigen Detection.
AED215Hu ELISA Kit for Anti-Chorionic Gonadotropin Beta Polypeptide Antibody (Anti-CGb) Enzyme-linked immunosorbent assay for Antibody Detection.
KSD215Hu01 ELISA Kit DIY Materials for Chorionic Gonadotropin Beta Polypeptide (CGb) Main materials for "Do It (ELISA Kit) Yourself".