ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3)
TNFAIP5; TSG-14; Pentaxin-Related Gene,Rapidly Induced By IL-1 Beta; Tumor Necrosis Factor Alpha-Induced Protein 5; Tumor necrosis factor-inducible gene 14 protein
- Product No.AEK411Hu
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Sample Typeserum, plasma and other biological fluids
- Test MethodDouble-antibody Sandwich
- Assay Length2h, 30min
- Detection Range31.2-2,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 13.3pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
For more details, please contact local distributors! US$ 920
For more details, please contact local distributors! US$ 4140
For more details, please contact local distributors! US$ 7820
For more details, please contact local distributors! US$ 64400
For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Anti-Pentraxin 3, Long Antibody (Anti-PTX3).
No significant cross-reactivity or interference between Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and analogues was observed.
Matrices listed below were spiked with certain level of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) in samples.
|Matrix||Recovery range (%)||Average(%)|
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Anti-Pentraxin 3, Long Antibody (Anti-PTX3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-Pentraxin 3, Long Antibody (Anti-PTX3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|TMB Substrate||1×9mL||Stop Solution||1×6mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 5 times;
5. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
6. Add 50µL Stop Solution. Read at 450nm immediately.
|Mediators of Inflammation||Biochemical Analysis of Pentraxin 3 and Fibrinogen Levels in Experimental Periodontitis Model PubMed: 22966213|
|Ibnosina Journal of Medicine and Biomedical Sciences||Effect of Vagus Nerve Stimulation on Focal Transient Cerebral Ischemia and Reperfusion in Adult Male White New Zealand Rabbits Sfu: Source|
|Hemodialysis International||Hemodialysis-induced changes in the blood composition affect function of the endothelium Onlinelibrary: hdi.12148|
|Hemodial Int.||Hemodialysis‐induced changes in the blood composition affect function of the endothelium Pubmed:24612443|
|Journal of Obstetrics and Gynaecology Research||The effects of sunitinib on endometriosis Pubmed:25093747|
|Gynecol Endocrinol.||Exenatide improves ovarian and endometrial injury and preserves ovarian reserve in streptozocin induced diabetic rats Pubmed:25366063|
|Taiwanese Journal of Obstetrics and Gynecology||Ovarian failure in diabetic rat model: Nuclear factor-kappaB, oxidative stress, and pentraxin-3 Pubmed:25510691|
|Journal of Obstetrics and Gynaecology Research||Octreotide protects ovary against ischemia–reperfusion injury in rats: Evaluation of histological and biochemical parameters PubMed: 26223286|
|J Obstet Gynaecol||The long pentraxin-3 is a useful marker for diagnosis of ovarian torsion: An experimental rat model PubMed: 26470829|
|Neurology Research International||Accumulation of α-Synuclein in Cerebellar Purkinje Cells of Diabetic Rats and Its Potential Relationship with Inflammation and Oxidative Stress Markers 5952149|
|Journal of Diabetes Research||Study on Association of Pentraxin 3 and Diabetic Nephropathy in a Rat Model Pubmed:29725602|
|World Journal of Cardiovascular Diseases||The Relationship between Serum Pentraxin-3 Levels and Severity of Coronary Heart Disease paperID:86206|
|Advances in Medical Sciences||Serum levels and gene expression of pentraxin 3 are elevated in COPD Pubmed: 30572222|
|BioMed Research International||Pentraxin 3 Detects Clinically Significant Fibrosis in Patients with Chronic Viral Hepatitis C|
|Evidence-Based Complementary and Alternative Medicine||Jie Du Tong Ye San Prevents N-Nitrosomethylbenzylamine-Induced Esophageal Carcinogenesis via Inhibition of Inflammation and Proliferation|
|Journal of Interferon and Cytokine Research||Study on Serum Pentraxin-3 Levels in Vasculitis with Hypertension Pubmed: 31264920|
|Scientific Reports||The role of CRP and Pentraxin 3 in the prediction of systemic inflammatory response syndrome and death in acute pancreatitis Pubmed: 31798002|
|J Obstet Gynaecol||Losartan ameliorates ovarian ischaemia/reperfusion injury in rats: an experimental study Pubmed: 31955629|
|J Pediatr Adolesc Gynecol||Effects of Methylene Blue on Ovarian TorsionLDetorsion Injury in a Rat Model Pubmed: 32593749|
|Acta Cirurgica Brasileira||Therapeutic effects of vitamin D on acetic acid-induced colitis in rats Pubmed: 32555936|
|Catalog No.||Related products for research use of Homo sapiens (Human) Organism species||Applications (RESEARCH USE ONLY!)|
|RPK411Hu01||Recombinant Pentraxin 3, Long (PTX3)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAK411Hu02||Polyclonal Antibody to Pentraxin 3, Long (PTX3)||WB; IHC; ICC; IP.|
|PAK411Hu01||Polyclonal Antibody to Pentraxin 3, Long (PTX3)||WB; IHC; ICC; IP.|
|MAK411Hu21||Monoclonal Antibody to Pentraxin 3, Long (PTX3)||WB; IHC; ICC; IP.|
|AEK411Hu||ELISA Kit for Anti-Pentraxin 3, Long Antibody (Anti-PTX3)||Enzyme-linked immunosorbent assay for Antibody Detection.|
|SEK411Hu||ELISA Kit for Pentraxin 3, Long (PTX3)||Enzyme-linked immunosorbent assay for Antigen Detection.|