Active Complement Component 5a (C5a)

ACTIVITY TEST

Complement Component 5a (C5a) is a component of the complement system which plays a key role in promoting migration and adherence of neutrophils and monocytes to vessel walls. C5a has been proven to be able to induce chemotactic migration of THP-1 cells. Therefore, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of C5a on the human monocytic cell line THP-1. Briefly, THP-1 cells were seeded into the upper chambers (100µL cell suspension, 106 cells/mL in RPMI 1640 with 0.5% FBS) and C5a (50ng/mL and 100ng/mL diluted separately in serum free RPMI 1640) was added in lower chamber with a polycarbonate filter (8µm pore size) used to separate the two compartments. After incubation at 37oC with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). Result: C5a is able to induce migration of THP-1 cells. The migrated THP-1 cells in low chamber at low magnification (×100) were shown in Figure 1. Five fields of each chamber were randomly chosen to count the migrated cells at high magnification (×400) and the statistical data was shown in Figure 2.
(A) THP-1 cells were seeded into the upper chambers and 50ng/mL C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h;
(B) THP-1 cells were seeded into the upper chambers and 100ng/mL C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h;
(C) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without C5a was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3h.
Figure. The chemotactic effect of C5a on THP-1 cells.

Figure. The chemotactic effect of C5a on THP-1 cells

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

INCREMENT SERVICES

Magazine Citations
Molecular Immunology Cathepsin D is released after severe tissue trauma in vivo and is capable of generating C5a in vitro ScienceDirect: S0161589011008297
Journal of Reproductive Immunology Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia PubMed: 23415845
The Journal of Clinical Investigation Complement component 5 contributes to poor disease outcome in humans and mice with pneumococcal meningitis PubMed: PMC3195471
Journal of Antimicrobial Chemotherapy Efficacy of a synthetic antimicrobial peptidomimetic versus vancomycin in a Staphylococcus epidermidis device-related murine peritonitis model Pubmed: 23645587
Journal of Orthopaedic Research Conversion from external fixator to intramedullary nail causes a second hit and impairs fracture healing in a severe trauma model Pubmed: 23070742
SpringerPlus Therapeutic effects of sesame oil on monosodium urate crystal-induced acute inflammatory response in rats Pubmed: 24353977
American Journal fo Reproductive Immunology Evaluation of the Markers of Inflammation in the Umbilical Cord Blood of Newborns of Mothers with Thrombophilia Pubmed:25209155
Gynecol Obstet Invest Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia? PubMed: 26485247
Journal of Neuroimmunology Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis Pubmed:27235358
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602
Digital Repository Dosagem de frações ativadas do sistema complemento em empiema induzido em ratos 10183
Environmental Pollution Cadmium-induced immune abnormality is a key pathogenic event in human and rat models of preeclampsia pubmed:27511439
Cancer Letters Complement C5a/C5aR pathway potentiates the pathogenesis Q5 of gastric cancer by down-regulating p21 expression pubmed:29031586
Acta Paediatrica Serum complement factor 5a levels are associated with nonalcoholic fatty liver disease in obese children DOI: 10.1111/apa.14106
Scientific Reports Splenectomy modulates early immuno-inflammatory responses to trauma-hemorrhage and protects mice against secondary sepsis Pubmed: 30291296
Nutrients The Beneficial Effects of Lactobacillus plantarum PS128 on High-Intensity, Exercise-Induced Oxidative Stress, Inflammation, and Performance in Triathletes Pubmed: 30736479
Journal of NeuroVirology Intrathecal complement activation by the classical pathway in tick-borne encephalitis Pubmed: 30850976
PLoS One Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model Pubmed: 31247004
Frontiers in Immunology C5a/C5aR Pathway is Critical for the Pathogenesis of Psoriasis Pubmed: 31447855
Phytomedicine Novel evidence that an alternative complement cascade pathway is involved in optimal mobilization of hematopoietic stem/progenitor cells in Nlrp3 …
Aging C5aR deficiency attenuates the breast cancer development via the p38/p21 axis Pubmed: 32669478
STEM CELL REVIEWS AND REPORTS A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem … Pubmed: 32406006
Modulation of C5a-C5aR interactions against murine mammary cancer cell line
FASEB J C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease Pubmed:35503088
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications (RESEARCH USE ONLY!)
RPA388Hu01 Recombinant Complement Component 5a (C5a) Positive Control; Immunogen; SDS-PAGE; WB.
APA388Hu62 Active Complement Component 5a (C5a) Cell culture; Activity Assays.
APA388Hu61 Active Complement Component 5a (C5a) Cell culture; Activity Assays.
EPA388Hu61 Eukaryotic Complement Component 5a (C5a) Positive Control; Immunogen; SDS-PAGE; WB.
PAA388Hu06 Polyclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
PAA388Hu01 Polyclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
MAA388Hu21 Monoclonal Antibody to Complement Component 5a (C5a) WB,IHC
MAA388Hu22 Monoclonal Antibody to Complement Component 5a (C5a) WB; IHC
MAA388Hu23 Monoclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
MAA388Hu24 Monoclonal Antibody to Complement Component 5a (C5a) WB; IHC; ICC; IP.
SEA388Hu ELISA Kit for Complement Component 5a (C5a) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA388Hu Multiplex Assay Kit for Complement Component 5a (C5a) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA388Hu01 ELISA Kit DIY Materials for Complement Component 5a (C5a) Main materials for "Do It (ELISA Kit) Yourself".