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Active Endostatin (ES)
- Product No.APA542Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 5% Trehalose.
- Traits Freeze-dried powder
- Purity> 90%
- Isoelectric Point9.1
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
- FOB
US$ 252
US$ 630
US$ 1260
US$ 3780
US$ 9450
For more details, please contact local distributors!
ACTIVITY TEST
Endostatin (ES) is a naturally occurring, 20kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. Endostatin is a broad-spectrum angiogenesis inhibitor and may interfere with the pro-angiogenic action of growth factors such as basic fibroblast growth factor (bFGF/FGF-2) and vascular endothelial growth factor (VEGF). To test the effect of ES on inhibit the VEGF basic-dependent proliferation of ECV304 endothelium cell line, cells were seeded into triplicate wells of 96-well plates at a density of 5, 000 cells/well when the cell attached, replaced with serum-free standard DMEM overnight. Then the medium was replaced with 2% serum standard DMEM which contain 10ng/mL of VEGFA and various concentrations of ES. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37℃. The result was shown in Figure 1. It was obvious that ES (100ng/mL) significantly inhibit cell proliferation of EV304 cells.
USAGE
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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| Magazine | Citations |
| Cancer Immunology | Endostatin gene therapy enhances the efficacy of IL-2 in suppressing metastatic renal cell carcinoma in mice SpringerLink: u38777121t6660l5 |
| Journal of Fluoresence | Erythrocyte Protoporphyrin Fluorescence as a Biomarker for Monitoring Antiangiogenic Cancer Therapy SpringerLink: 4v306130302257w0 |
| The Journal of Gene Medicine | Endostatin- and interleukin-2-expressing retroviral bicistronic vector for gene therapy of metastatic renal cell carcinoma Wiley: source |
| Biomedicine & Pharmacotherapy | Endostatin neoadjuvant gene therapy extends survival in an orthotopic metastatic mouse model of renal cell carcinoma ScienceDirect: S075333221100151X |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats Scialert: Source |
| The Journal of Immunology | Inflammation Induced by MMP-9 Enhances Tumor Regression of Experimental Breast Cancer PubMed: PMC3619527 |
| Prostaglandins & Other Lipid Mediators | Promotion of adipogenesis by an EP2 receptor agonist via stimulation of angiogenesis in pulmonary emphysema Pubmed:24911647 |
| American Journal of Physiology - Heart and Circulatory Physiology | Endostatin and kidney fibrosis in aging: a case for antagonistic pleiotropy? Source |
| shock | Angiogenesis inhibitor endostatin protects mice with sepsis from multiple organ dysfunction syndrome PubMed: 26125086 |
| Clin Rev Allergy Immunol | Endothelial Dysfunction and Nailfold Videocapillaroscopy Pattern as Predictors of Digital Ulcers in Systemic Sclerosis: a Cohort Study and Review of the Literature PubMed: 26142066 |
| Angiologia e Cirurgia Vascular | Peripheral vasculopathy in Raynaud phenomenon: Vascular disease biomarkers science:S1646706X16300052 |
| Clinical Rheumatology | Impaired angiogenesis as a feature of digital ulcers in systemic sclerosis Pubmed:26920752 |
| Blood biomarker for early blood brain barrier disruption in ischemic stroke patent:US20170199205A1 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| APA542Hu61 | Active Endostatin (ES) | Cell culture; Activity Assays. |
| EPA542Hu61 | Eukaryotic Endostatin (ES) | Positive Control; Immunogen; SDS-PAGE; WB. |
| CPA542Hu31 | KLH Conjugated Endostatin (ES) | Immunogen; SDS-PAGE; WB. |
| PAA542Hu01 | Polyclonal Antibody to Endostatin (ES) | WB; IHC; ICC; IP. |
| PAA542Hu06 | Polyclonal Antibody to Endostatin (ES) | WB; IHC; ICC; IP. |
| WEA542Hu | Wide-range ELISA Kit for Endostatin (ES) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA542Hu | ELISA Kit for Endostatin (ES) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA542Hu | ELISA Kit for Endostatin (ES) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA542Hu | Multiplex Assay Kit for Endostatin (ES) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA542Hu11 | ELISA Kit DIY Materials for Endostatin (ES) | Main materials for "Do It (ELISA Kit) Yourself". |