CLIA Kit for Corticosterone (Cort)
- Product No.CCA540Ge
- Organism SpeciesPan-species (General) Same name, Different species.
- Sample TypeSerum, plasma, urine and other biological fluids
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range781-200,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 275pg/mL.
- DownloadInstruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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For more details, please contact local distributors!
This assay has high sensitivity and excellent specificity for detection of Corticosterone (Cort).
No significant cross-reactivity or interference between Corticosterone (Cort) and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Corticosterone (Cort) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Corticosterone (Cort) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
|Pre-coated, ready to use 96-well strip plate||1||Plate sealer for 96 wells||4|
|Detection Reagent A||1×120µL||Assay Diluent A||1×12mL|
|Detection Reagent B||1×120µL||Assay Diluent B||1×12mL|
|Substrate A||1×10mL||Substrate B||1×2mL|
|Wash Buffer (30 × concentrate)||1×20mL||Instruction manual||1|
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
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|EXPERIMENTAL & CLINICAL CARDIOLOGY||Sick Sinus Syndrome May Attribute to the Decrease of ?±-klotho Protein in Mice Com:Source|
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|Behav Brain Res||Rats with differential self-grooming expression in the elevated plus-maze do not differ in anxiety-related behaviors PubMed: 26142783|
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|Respiratory physiology & neurobiology||Curcumin attenuates chronic intermittent hypoxia-induced brain injuries by inhibiting AQP4 and p38 MAPK pathway Pubmed:29758366|
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|Brain, Behavior, and Immunity||HMGB1-Mediated Differential Response on Hippocampal Neurotransmitter Disorder and Neuroinflammation in Adolescent Male and Female Mice following Cold Exposure Pubmed: 30476565|
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|Scientific Reports||Mice lacking Casp1, Ifngr and Nos2 genes exhibit altered depressive-and anxiety-like behaviour, and gut microbiome composition Pubmed: 31015500|
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